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ナイシンA作用により分離したナイシンA高度耐性MRSAのビルレンスに及ぼす影響
http://hdl.handle.net/10232/00031133
http://hdl.handle.net/10232/000311335b90b803-aea4-49fb-bfd2-45bc75719381
名前 / ファイル | ライセンス | アクション |
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Diss_渡邉_温子_ISK561_2020 (1.6 MB)
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Abstract_渡邉_温子_4516800196_2019 (228.6 kB)
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Result_Watanabe_Atsuko_isk_561 (988.8 kB)
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Comments_Watanabe_Atsuko_isk_561 (541.1 kB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2020-07-08 | |||||
タイトル | ||||||
タイトル言語 | en | |||||
タイトル | An alternative nisin A resistance mechanism affects virulence in Staphylococcus aureus | |||||
タイトル | ||||||
タイトル言語 | ja | |||||
タイトル | ナイシンA作用により分離したナイシンA高度耐性MRSAのビルレンスに及ぼす影響 | |||||
著者 |
渡邉, 温子
× 渡邉, 温子 |
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言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
要約(Abstract) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nisin A is a bacteriocin produced by Lactococcus lactis and is widely used as a food preservative. Staphylococcus aureus has the BraRS-VraDE system that provides resistance against low concentrations of nisin A. BraRS is a two-component system that induces the expression of the ABC transporter VraDE. Previously, we isolated a highly nisin A-resistant strain with increased VraDE expression due to a mutation in braRS. In this study, we isolated S. aureus MW2 mutants with BraRS-VraDE-independent nisin A resistance. These mutants, designated SAN2 (S. aureus nisin resistant) and SAN469, had a mutation in pmtR, which encodes a transcriptional regulator responsible for the expression of the pmtABCD operon. As a result, these mutants exhibited increased expression of PmtABCD, a transporter responsible for the export of phenol-soluble modulin (PSM). Characterization of the mutants revealed that they have decreased susceptibility to human β-defensin-3 (hBD3) and LL37, which are innate immune factors. Additionally, these mutants showed higher hemolytic activity than the original MW2 strain. Furthermore, in a mouse bacteremia model, the SAN2 strain exhibited a lower survival rate than the original MW2 strain. These results indicate that the increased expression of pmtABCD due to a pmtR mutation is an alternative nisin A resistance mechanism that also affects virulence in S. aureus. Miki Kawada-Matsuo, Atsuko Watanabe, Kaoru Arii, Yuichi Oogai, Kazuyuki Noguchi, Shouichi Miyawaki, Tetsuya Hayashi, Hitoshi Komatsuzawa Staphylococcus aureus Virulence Affected by an Alternative Nisin A Resistance Mechanism Applied and Environmental Microbiology 2020 DOI: 10.1128/AEM.02923-19 |
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内容記述言語 | en | |||||
作成日 | ||||||
日付 | 2020-04-02 | |||||
日付タイプ | Collected | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
NDC | ||||||
主題Scheme | NDC | |||||
主題 | 490 | |||||
ファイル(説明) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨 | |||||
言語 | ja | |||||
公開者・出版者 | ||||||
出版者言語 | en | |||||
出版者 | American Society for Microbiology | |||||
公開者・出版者 | ||||||
出版者言語 | ja | |||||
出版者 | 鹿児島大学 | |||||
公開者・出版者 | ||||||
出版者言語 | ja-Kana | |||||
出版者 | カゴシマ ダイガク | |||||
備考 | ||||||
備考 | 【指導教員:宮脇正一】 | |||||
date.appl | ||||||
【学位申請日】2020-03-04 | ||||||
学位名 | ||||||
学位名の言語 | ja | |||||
学位名 | 博士(歯学) | |||||
学位名 | ||||||
学位名の言語 | en | |||||
学位名 | Doctor of Philosophy in Dental Science | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 17701 | |||||
学位授与機関名の言語 | ja | |||||
学位授与機関名 | 鹿児島大学 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2020-04-20 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲総研第561号 |