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家畜・家禽のゲノムDNA抽出におけるガラスろ紙法の検討
http://hdl.handle.net/10232/9019
http://hdl.handle.net/10232/901912b4aefc-24f8-4564-a4fc-4f9a5df78396
名前 / ファイル | ライセンス | アクション |
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AN00040636_v32_p13-17.pdf (1.3 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2015-01-07 | |||||
タイトル | ||||||
タイトル | 家畜・家禽のゲノムDNA抽出におけるガラスろ紙法の検討 | |||||
タイトルよみ | ||||||
タイトルよみ | カチク・カキン ノ ゲノム DNA チュウシュツ ニ オケル ガラス ロシホウ ノ ケントウ | |||||
別言語のタイトル | ||||||
その他のタイトル | Examination of the Method of Glass Filter Paper in the Genomic DNA Extraction of Domestic Animal/Poultry | |||||
著者 |
工藤, 美雪
× 工藤, 美雪× 河邊, 弘太郎× 大久津, 昌治× 岡本, 新× 山口, 浩× 安江, 博× 前田, 芳實× 下桐, 猛 |
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著者よみ | ||||||
姓名 | クドウ, ミユキ | |||||
著者よみ | ||||||
姓名 | カワベ, コウタロウ | |||||
著者よみ | ||||||
姓名 | オオクツ, ショウジ | |||||
著者よみ | ||||||
姓名 | オカモト, シン | |||||
著者よみ | ||||||
姓名 | ヤマクチ, ヒロシ | |||||
著者よみ | ||||||
姓名 | ヤスエ, ヒロシ | |||||
著者よみ | ||||||
姓名 | マエダ, ヨシザネ | |||||
著者よみ | ||||||
姓名 | シモギリ, タケシ | |||||
別言語の著者 | ||||||
姓名 | Kudo, Miyuki | |||||
別言語の著者 | ||||||
姓名 | Kawabe, Kotaro | |||||
別言語の著者 | ||||||
姓名 | Ookutsu, Shoji | |||||
別言語の著者 | ||||||
姓名 | Okamoto, Shin | |||||
別言語の著者 | ||||||
姓名 | Yamakuchi, Hiroshi | |||||
別言語の著者 | ||||||
姓名 | Yasue, Hiroshi | |||||
別言語の著者 | ||||||
姓名 | Maeda, Yoshizane | |||||
別言語の著者 | ||||||
姓名 | Shimogiri, Takeshi | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 安価 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ガラス繊維ろ紙 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ゲノムDNA抽出 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 血球 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | blood cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cost-effective | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | genomic DNA purification | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | glass fiber filter | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
要約(Abstract) | ||||||
内容記述タイプ | Other | |||||
内容記述 | In this study, to develop a cost-effective and rapid genomic DNA purification method in animals and birds, we examined the DNA purification method using glass fiber filter proven in plant genomics. Four or eight blood cell samples from each of cattle and chickens were used for the purification. The purification method was basically the same as that described previously (Muramoto et al. 2005). Several points were tested in this method for recovering DNA efficiently, revealing the following: 1)One μg of genomic DNA was purified from the chicken blood cell samples diluted 50-200 times. It could be used for -5kb PCR amplification. Genomic DNA from the chicken blood cell samples diluted 1-10 times could not be used for PCR amplification. In cattle, genomic DNA was purified from the blood cell samples diluted 1-200 times. It could be use for 0.8kb PCR amplification. 2)Concerning concentration of sodium sulfite in sample storage, 10% was acceptable. 3)Drying the filter with the blood cells before purifying improved DNA yield. 4)Conversion from sodium sulfite to vitamin C failed to obtain the genomic DNA from the samples. 5)When the filter with the blood cells was stored for four weeks at room temperature, genomic DNA was purified. We purified the genomic DNAs using this method for 10 yen/sample and from 16 samples at 1 hour. These results suggest that this method is a cost-effective and rapid genomic DNA purification method in animals and birds. | |||||
収録雑誌名 |
鹿児島大学農学部農場研究報告=Bulletin of the Experimental Farm Faculty of Agriculture,Kagoshima University 巻 32, p. 13-17 |
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作成日 | ||||||
日付 | 2010-03-16 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
ISSN | 03860132 | |||||
NII書誌ID(雑誌) | ||||||
収録物識別子タイプ | NCID | |||||
NC ID | AN00040636 | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
NDC | ||||||
主題Scheme | NDC | |||||
主題 | 640 | |||||
公開者・出版者 | ||||||
出版者 | 鹿児島大学 | |||||
公開者別名 | ||||||
公開者別名 | Kagoshima University |