2026-03-05T20:23:53Z https://ir.kagoshima-u.ac.jp/oai

oai:ir.kagoshima-u.ac.jp:00015984 2026-03-03T09:41:33Z 228:262:6034 39:40

Eel Serum Synergistically Enhances Growth of Fish Cell Line GAKS TAKUMI, Shota KOMATSU, Yumiko DOMEN, Yasuhito SUMIZAWA, Tomoyuki SHIOZAKI, Kazuhiro ISHIKAWA, Manabu KOMATSU, Masaharu HAYASHI, Seiichi YAMADA, Shoji open access cell culture eel serum fish cell line GAKS Generation of cell lines derived from fish is getting to increase. Generally, mammalian cell line culture needs appropriate media with approximately 5 to 10 % fetal calf serum. However, the optimal culture conditions for fish cell lines have not been established so far. We investigated the effect of exposure to inactivated fish serum on cell culture of goldfish scale cell line GAKS. The 5 % sera from gibel, carp, tilapia, and eel were toxic to GAKS cells. In addition, these fish serum were toxic to not only the GAKS, but also the mammalian cell lines HEK293 and SH-SY5Y. GAKS cells did not proliferate under the culture condition with 5% fish serum. Although it is known that fish serum contains thiaminase, the effect of supplemental addition of vitamin B1 was negligible for the proliferation of GAKS cells. Lower concentrations of fish sera, especially less than 5 % of eel serum stimulated the cell survival of GAKS compared with 5 % of each fish serum. The culture medium containing around 1 % eel serum coupled with 1% FCS was comparable to 5 % FCS conditions for proliferation of GAKS cells. Furthermore, the passage culture was successful under these culture conditions. 鹿児島大学 Kagoshima University 2021 eng departmental bulletin paper VoR http://hdl.handle.net/10232/00031871 https://ir.kagoshima-u.ac.jp/records/15984 0453087X AN00040498 鹿児島大学水産学部紀要 Memoirs of Faculty of Fisheries Kagoshima University 70 1 8 https://ir.kagoshima-u.ac.jp/record/15984/files/AN00040498_70_p1-8.pdf application/pdf 1.3 MB 2022-04-08 2021