2026-03-14T03:14:34Z https://ir.kagoshima-u.ac.jp/oai

oai:ir.kagoshima-u.ac.jp:00016087 2026-03-03T14:57:11Z 57:86

Impact of Oncogenic Targets Controlled by Tumor-Suppressive miR-30a-5p in Pancreatic Ductal Adenocarcinoma miR-30a-5pによって制御される癌促進的分子が膵管腺癌に及ぼす影響 Nepal, Pramod ネパール, プラモド open access microRNA miR-30a-5p RRM2 pancreatic ductal adenocarcinoma tumor-suppressor 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨 Background/Aim: Our recent miRNA analyses revealed that miR-30a-5p has tumor-suppressive activity in pancreatic ductal adenocarcinoma (PDAC). Herein, we sought to identify tumor-suppressive genes controlled by miR-30a-5p, emphasizing on genes that are closely involved in the molecular pathogenesis of PDAC. We uncovered several novel findings regarding the pathogenesis of this disease. Materials and Methods: In silico analyses were used to identify the putative target genes of miR-30a-5p and assess their expression levels. Direct regulation of RRM2 by miR-30a-5p and its oncogenic functions were evaluated in PDAC cell lines. Overexpression of RRM2 was demonstrated in clinical samples. Results: A total of 24 putative targets were identified by in silico database analysis. High expression of 4 genes (CBFB, RRM2, AHNAK, and DCBLD1) was significantly associated with shorter survival of patients with PDAC. Functional assays demonstrated that knockdown of RRM2 attenuated the malignant phenotype of PDAC cells. Conclusion: The miR-30a-5p/RRM2 axis facilitated the malignant transformation of PDAC cells. PRAMOD NEPAL, YUTO HOZAKA, TAKAKO TANAKA, MASUMI WADA, SHUNICHI ASAI, CHIKASHI MINEMURA, TETSUYA IDICHI, TAKAAKI ARIGAMI, HIROSHI KURAHARA, NAOHIKO SEKI and TAKAO OHTSUKA Impact of Oncogenic Targets Controlled by Tumor-Suppressive miR-30a-5p in Pancreatic Ductal Adenocarcinoma ANTICANCER RESEARCH 41: 4821-4836 (2021) https://doi.org/10.21873/anticanres.15297 International Institute of Anticancer Research 鹿児島大学 eng doctoral thesis VoR http://hdl.handle.net/10232/00031973 https://ir.kagoshima-u.ac.jp/records/16087 甲総研第635号 博士(医学) Doctor of Philosophy in Medical Science 2022-02-17 17701 鹿児島大学 https://ir.kagoshima-u.ac.jp/record/16087/files/Diss_プラモド_ネパール_ISK635_2021.pdf application/pdf 5.3 MB 2022-06-21 2022-02-07 https://ir.kagoshima-u.ac.jp/record/16087/files/Abstract_プラモド_ネパール_4517810437_2021.pdf application/pdf 326.7 kB 2022-06-24 2022-02-07 https://ir.kagoshima-u.ac.jp/record/16087/files/Result_Pramod_Nepal_isk_635.pdf application/pdf 146.7 kB 2022-06-06 2022-02-07 https://ir.kagoshima-u.ac.jp/record/16087/files/Comments_Pramod_Nepal_isk_635.pdf application/pdf 67.8 kB 2022-06-06 2022-02-07