@techreport{oai:ir.kagoshima-u.ac.jp:00001027,
 author = {宮下, 圭一},
 month = {2016-10-27},
 note = {2011-2012年度科学研究費助成事業(科学研究費補助金(若手研究(B))研究成果報告書　課題番号：23791911　研究代表者：宮下圭一(鹿児島大学・医学部・歯学部附属病院・助教), マウスのアレルギー性鼻炎モデルを用いて、Th1/Th2バランスおよびアレルギー病態に対するPCの関与について検討した。OVA感作前にPC-BSAを経鼻投与し、アレルギー症状、鼻粘膜への好酸球浸潤、血清IgE値、脾臓のCD4陽性T細胞のサイトカイン産生能を検討した。PC-BSAの経鼻前投与によりくしゃみ、鼻掻き回数が減少し、血清中OVA特異的IgE濃度の低下を認めた。さらに脾臓CD4陽性T細胞のIL-4産生の減少が認められた。これらの結果からマウスのPC前投与のアレルギー性鼻炎に対する抑制効果が示唆され、PCにはTh2型のアレルギー反応を抑制する働きがあると考えられた。, Background: Phosphorylcholine (PC), which is a structural component of wide variety of bacteria. However immunomodulatory properties of PC in the upper respiratory tract have not yet been characterized. Objective: We sought to elucidate the effects of PC on the regulation of allergic response in the upper respiratory tract. Methods: Allergic rhinitis model established by ovalbumin (OVA) sensitization was pretreated by intranasal or peritoneal administration of PC-bovine serum albumin (PC-BSA). Allergic responses were compared with non-pretreated mice. IL-12p40 production by DCs stimulated with PC was measured to evaluate the effect of PC on the TH2 responses mediated by DCs. Results: PC-pretreated mice showed mild allergic nasal symptoms compared to the mice without PC-pretreatment. The levels of total IgE and OVA-specific IgE were significantly lower in PC-pretreated mice. The IL-4 production by splenic CD4+ T cells was down-regulated by PC-pretreatment. Further, PC induced IL-12p40 production by DCs. Conclusion: These findings indicate that PC inhibits the allergic response in the upper respiratory tract mediated by the induction of IL-12p40 production by DCs. Thus, PC might be useful as an immunotherapeutic agent for allergic diseases.},
 title = {粘膜ワクチンによるⅠ型アレルギーの誘導機序とその制御に関する研究},
 year = {}
}