@article{oai:ir.kagoshima-u.ac.jp:00010379,
 author = {UMATA, Hidetaka and 馬田, 英隆 and KUBOTA, Katsuyoshi and 久保田, 勝義 and INOUE, Sachiko and 井上, 幸子 and SHIIBA, Yasuki and 椎葉, 康喜 and NAGASAWA, Hisami and 長澤, 久視},
 journal = {鹿児島大学農学部演習林研究報告, Research bulletin of the Kagoshima University forests},
 month = {Dec},
 note = {Galeola septentrionalis is an achlorophyllous myco-heterotrophic orchid and has been known to establish a symbiotic association with several biological species of Armillariella mellea (Basidiomycetes). However，there have been no successful reports elucidating an association between a mycobiont and seeds of this orchid during germination. In this study，a field sowing experiment using seeds from this orchid was conducted for more than 14 months in the natural habitat of this species. Approximately 0.1% of the seeds germinated as the enlarging embryos ruptured the outer seed coat. Seeds that failed to germinate were collected and cultured in vitro on an agar medium containing mannitol，yeast extract，and other nutrients and incubated at 27.5℃. Approximately 4% of the seeds germinated six weeks after sowing. In this case，however，the two valves of the seed coat opened to release the enlarged embryo. The embryos of germinating seeds　were devoid of fungal pelotons. Seed germination appears to take place in the absence of mycobiont infestation in this　specles., ツチアケビは無葉緑の菌寄生性のラン科植物で，ナラタケ菌の生物学的種の数種と共生することが知られている。しかしながら，これまで本種の種子がその発芽の過程の中でナラタケ薗と共生したという報告はない。本研究では，ツチアケビ種子を母植物の発生地に14ヶ月間以上埋設する試験を行った。その結果，約0.1%の稜子が外種皮を破って発芽していた。また，その際に回収した未発芽種子をマンニット，精製酵母などを含む培地で27.5℃で培養すると，約4%の種子が外種皮の殻を開いて発芽した。発芽した種子の胚の内部に菌球は見あたらなかった。ツチアケビの種子においては共生菌の侵入がなくても発芽するものと思われる。},
 pages = {69--74},
 title = {Naturally germinating seeds of the achlorophyllous orchid Galeola septentrionalis contained no fungal pelotons},
 volume = {34},
 year = {2006}
}