{"created":"2023-07-25T08:10:40.251050+00:00","id":10649,"links":{},"metadata":{"_buckets":{"deposit":"9b03c9ce-2f34-43ca-a9bf-4418f7ddb655"},"_deposit":{"created_by":3,"id":"10649","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10649"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00010649","sets":[]},"author_link":["118049","118050","42041"],"item_8_alternative_title_18":{"attribute_name":"別言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Regulation of L-type Ca^{2+} channel activity by calmodulin"}]},"item_8_alternative_title_19":{"attribute_name":"タイトルよみ","attribute_value_mlt":[{"subitem_alternative_title":"Lガタ Caチャネル ノ カッセイ セイギョ ニ オケル カルモジュリン ノ ヤクワリ ト ソノ チョウセツ キコウ"}]},"item_8_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2011-03-31"}]},"item_8_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"2009-2010年度科学研究費補助金(若手研究(B))研究成果報告書 課題番号：21790206　研究代表者：蓑部悦子 (鹿児島大学医歯学総合研究科助教)","subitem_description_type":"Other"},{"subitem_description":"カルモジュリンによるL型Caチャネルの調節機構を解明することを目的とし、電気生理学的手法を用いて、チャネル変異体の活性を解析した。結果から、チャネルの活性化にはカルモジュリンの結合が必須であること、野生型ではカルモジュリンに加えATPが必要であるのに対し、変異体ではカルモジュリンのみで活性を示すこと、カルモジュリンとATPがチャネルC末端近位部に結合し、その作用はC末端遠位部により抑制されることが示唆された。","subitem_description_type":"Other"},{"subitem_description":"We measured activity of L-type Ca^{2+} channel and its mutants, C-terminal deletion (delta CT) and delta CT linked with calmodulin (CaM), using patch-clamp technique (inside-out mode). We found that binding of CaM to the channel was required for the channel activity. Unlike the wild type channel, the mutants did not require ATP for the channel activity. It was suggested that CaM and ATP interacted with a proximal part of C-terminal thereby producing the channel activity, which was modulated negatively by a distal part of C-terminal.","subitem_description_type":"Other"}]},"item_8_full_name_2":{"attribute_name":"著者よみ","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"ミノベ, エツコ"}]}]},"item_8_full_name_3":{"attribute_name":"別言語の著者","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"MINOBE, Etsuko"}]}]},"item_8_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学"}]},"item_8_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"491","subitem_subject_scheme":"NDC"}]},"item_8_text_24":{"attribute_name":"公開者よみ","attribute_value_mlt":[{"subitem_text_value":"カゴシマ ダイガク"}]},"item_8_text_25":{"attribute_name":"公開者別名","attribute_value_mlt":[{"subitem_text_value":"Kagoshima University"}]},"item_8_text_41":{"attribute_name":"科研費番号 ","attribute_value_mlt":[{"subitem_text_value":"21790206"}]},"item_8_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"蓑部, 悦子"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-28"}],"displaytype":"detail","filename":"21790206seika.pdf","filesize":[{"value":"187.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"21790206seika.pdf","url":"https://ir.kagoshima-u.ac.jp/record/10649/files/21790206seika.pdf"},"version_id":"637d07a2-5512-4cbf-b0cd-4dd879b86443"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"カルシウムチャネル","subitem_subject_scheme":"Other"},{"subitem_subject":"カルモジュリン","subitem_subject_scheme":"Other"},{"subitem_subject":"パッチクランプ法","subitem_subject_scheme":"Other"},{"subitem_subject":"inside-out","subitem_subject_scheme":"Other"},{"subitem_subject":"run-down","subitem_subject_scheme":"Other"},{"subitem_subject":"カルシウムイオン","subitem_subject_scheme":"Other"},{"subitem_subject":"ATP","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"L型Caチャネルの活性制御におけるカルモジュリンの役割とその調節機構","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"L型Caチャネルの活性制御におけるカルモジュリンの役割とその調節機構"}]},"item_type_id":"8","owner":"3","path":["80"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-01-06"},"publish_date":"2015-01-06","publish_status":"0","recid":"10649","relation_version_is_last":true,"title":["L型Caチャネルの活性制御におけるカルモジュリンの役割とその調節機構"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-12-25T07:18:57.878863+00:00"}