{"created":"2023-07-25T08:10:44.576466+00:00","id":10746,"links":{},"metadata":{"_buckets":{"deposit":"a4307ce3-e7cb-4188-a5f9-5211c701812c"},"_deposit":{"created_by":3,"id":"10746","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10746"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00010746","sets":["228:262:4831"]},"author_link":["104533","104526","104534","104527","104532","104528"],"item_7_alternative_title_18":{"attribute_name":"別言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"海洋細菌のプロテアーゼに関する研究 III : プロテアーゼの精製と酵素学的性質について"}]},"item_7_biblio_info_5":{"attribute_name":"収録雑誌名","attribute_value_mlt":[{"bibliographicPageEnd":"78","bibliographicPageStart":"71","bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"鹿児島大学水産学部紀要=Memoirs of Faculty of Fisheries Kagoshima University"}]}]},"item_7_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"1977-12-25"}]},"item_7_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Four components with casein hydrolysing activity were found in extracellular protease produced by a marine Pseudomonas sp. I-6. Each protease component was fractionated by use\nof DEAE-Sephadex A-50, Sephadex G-100 and polyacrylamide gel electrophoresis. Protease\nactivity was determined by the digestion of casein in the reaction mixture for fractions obtained\non column chromatography and on a casein agar plate for polyacrylamide gels, respectively.\nThe method of a casein agar plate was found to be convenient to detect the protease activity\non the gel.\nProtease fractions were alkaline proteases of which optimal pHs were from pH 10.0 to pH\n11.0. Proteases F-IIa and F-IIb were very low in activity without Ca^++ ion but reactivated\nwith the addition of Ca^++ ion remarkably, whereas the activities of F-Ia and F-Ib were unaffected or slightly affected by Ca^++ ion. Proteases of this strain were stabilized to heat by\nCa^++ ion but no substantial decrease in activity was observed during 6 hr incubation at 25 C\nregardless of the absence of Ca^++ ion.","subitem_description_type":"Other"}]},"item_7_full_name_3":{"attribute_name":"別言語の著者","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"坂田, 泰造"}]},{"nameIdentifiers":[{}],"names":[{"name":"住吉, 和郎"}]},{"nameIdentifiers":[{}],"names":[{"name":"柿本, 大壱"}]}]},"item_7_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学"}]},"item_7_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0453087X","subitem_source_identifier_type":"ISSN"}]},"item_7_source_id_9":{"attribute_name":"NII書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00040498","subitem_source_identifier_type":"NCID"}]},"item_7_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"663","subitem_subject_scheme":"NDC"}]},"item_7_text_24":{"attribute_name":"公開者よみ","attribute_value_mlt":[{"subitem_text_value":"カゴシマ ダイガク"}]},"item_7_text_25":{"attribute_name":"公開者別名","attribute_value_mlt":[{"subitem_text_value":"Kagoshima University"}]},"item_7_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"SAKATA, Taizo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SUMIYOSHI, Kazuro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KAKIMOTO, Daiichi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-31"}],"displaytype":"detail","filename":"AN00040498_v26_p71-78.pdf","filesize":[{"value":"992.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN00040498_v26_p71-78.pdf","url":"https://ir.kagoshima-u.ac.jp/record/10746/files/AN00040498_v26_p71-78.pdf"},"version_id":"02a82d8f-4465-4f33-bd13-fabe6ddd5cda"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Studies on the Proteases of Marine Bacteria III : Purification and Some Enzymatic Properties of Proteases","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Studies on the Proteases of Marine Bacteria III : Purification and Some Enzymatic Properties of Proteases"}]},"item_type_id":"7","owner":"3","path":["40","4831"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-04-29"},"publish_date":"2012-04-29","publish_status":"0","recid":"10746","relation_version_is_last":true,"title":["Studies on the Proteases of Marine Bacteria III : Purification and Some Enzymatic Properties of Proteases"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2024-01-30T05:45:47.179456+00:00"}