@article{oai:ir.kagoshima-u.ac.jp:00010825,
 author = {日高, 富男 and 一田, 謙一},
 issue = {1},
 journal = {鹿児島大学水産学部紀要=Memoirs of Faculty of Fisheries Kagoshima University},
 month = {2016-10-31},
 note = {In the experiments of bacteriophage, firstly, it is necessary to remove completely the large numbers of bacteria present in the samples or the phage-lysates without affecting unduly any phage which may have been present. Sterilization of them by filtration through membrane filters (average pore diameter, 0.45μ) is most commonly used for this purpose. The authors observed the following undesirable effects of filtration through membrane filters on some marine bacteriophages. 1) The phage particles stuk to the membrane and considerable or even complete loss of phage may occur, particularly if titers are low. 2) The marine phages are inactivated by a very small amount of surface active agents contained in a membrane filter. The surfactants act differently on different phages. These undesirable effecfs are avoided by prewashing membrane filters with sea water broth.},
 pages = {97--102},
 title = {海洋バクテリオファージの細菌濾過膜通過による損失},
 volume = {21},
 year = {}
}