@article{oai:ir.kagoshima-u.ac.jp:00010862,
 author = {OOSHIRO, Zentaro and YU, Fu-gong and HAYASHI, Seiichi and HINO, Koichi and KOJIMA, Hirotada},
 journal = {鹿児島大学水産学部紀要=Memoirs of Faculty of Fisheries Kagoshima University},
 month = {2016-10-31},
 note = {High density lipoprotein (HDL) with density between 1.063 and 1.21 g/ml was isolated from
eel serum by sequential floatation ultracentrifugation. Apolipoprotein was purified from
delipidated HDL by gel filtration on a Toyopearl HW-55F column in the presence of urea. Two
main apolipoproteins, designated as apo-A I and apo-A II, were obtained. Apo-A I was
purified as a single protein with a molecular weight of 28,000. Apo-A II had a molecular weight
of 14,000 and its purity was over 90%.
Synthesis and secretion of apolipoprotein of HDL by using ^{14}C-leucine was studied with
cultured eel-hepatocytes. It was demonstrated that cultured eel-hepatocytes synthesized and
secreted both apo-A I and apo-A II. We found that cells cultured for 9 days secreted more
proteins among cells cultured for 12 days.},
 pages = {25--33},
 title = {Apolipoprotein of High Density Lipoprotein of Eel Serum},
 volume = {37},
 year = {}
}