{"created":"2023-07-25T08:11:00.871778+00:00","id":11096,"links":{},"metadata":{"_buckets":{"deposit":"99e24e52-aac2-43da-bc34-c96d6da489ba"},"_deposit":{"created_by":18,"id":"11096","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"11096"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00011096","sets":["43:81"]},"author_link":["2619"],"item_8_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2012-01-25","subitem_date_issued_type":"Collected"}]},"item_8_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"2008-2010年度科学研究費補助金(基盤研究(C))研究成果報告書　課題番号：20580035　研究代表者：山本雅史 (鹿児島大学農学部准教授)","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"ナシにおけるゲノム研究を利用した画期的な育種技術の開発のため、遺伝子の担体である染色体について研究を実施した。概要は以下の通りである。（１）酵素解離法による良好な染色体標本作製技術を確立した。（２）蛍光色素のCMAおよびDAPIを用いた分染によって染色体構成を明らかにした。（３）FISH（蛍光 in situ ハイブリダイゼーション）によりリボゾームおよびレトロトランスポゾン遺伝子の染色体上での位置を決定した。","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"Studies of chromosome were conducted to progress breeding of pear through genome studies. The results are as follows; (1) Preparation of good chromosome sample using enzymatic maceration and air drying method was established, (2) The chromosome configuration was elucidated by banding technique using fluorochrome of CMA and DAPI, (3) The gene sites of rDNA and retrotransposons on chromosomes were detected  by FISH (fluorescent in situ hybridization).","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_8_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"Kagoshima University","subitem_publisher_language":"en"}]},"item_8_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"625","subitem_subject_scheme":"NDC"}]},"item_8_text_41":{"attribute_name":"科研費番号 ","attribute_value_mlt":[{"subitem_text_value":"20580035"}]},"item_8_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山本, 雅史","creatorNameLang":"ja"},{"creatorName":"ヤマモト, マサシ","creatorNameLang":"ja-Kana"},{"creatorName":"YAMAMOTO, Masashi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2619","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000000305161","nameIdentifierScheme":"NRID","nameIdentifierURI":" "}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-31"}],"displaytype":"detail","filename":"20580035seika.pdf","filesize":[{"value":"230.1 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"20580035seika.pdf","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/11096/files/20580035seika.pdf"},"version_id":"c4cb7ac7-d15f-470f-9746-ab3fe565a97b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"CMA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"FISH","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"rDNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"蛍光染色","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"ゲノム","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"染色体","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"ナシ","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"レトロトランスポゾン","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ナシにおける染色体識別と染色体上における有用遺伝子の可視化","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ナシにおける染色体識別と染色体上における有用遺伝子の可視化","subitem_title_language":"ja"},{"subitem_title":"Karyotyping and visualization of chromosomal sites of useful genes in pear","subitem_title_language":"en"}]},"item_type_id":"8","owner":"18","path":["81"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2014-12-25"},"publish_date":"2014-12-25","publish_status":"0","recid":"11096","relation_version_is_last":true,"title":["ナシにおける染色体識別と染色体上における有用遺伝子の可視化"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-04-24T07:02:52.578355+00:00"}