{"created":"2023-07-25T08:11:43.659034+00:00","id":11966,"links":{},"metadata":{"_buckets":{"deposit":"31ef8f3b-3066-43a2-a4db-be50f81d73de"},"_deposit":{"created_by":3,"id":"11966","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"11966"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00011966","sets":[]},"author_link":["106330","32293","106331"],"item_8_alternative_title_18":{"attribute_name":"別言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Osteochondral regeneration with scaffold free 3D-structure of swine adipose tissue-derived mesenchymal stem cells."}]},"item_8_alternative_title_19":{"attribute_name":"タイトルよみ","attribute_value_mlt":[{"subitem_alternative_title":"コツ ナンコツ ケッソン モデル ドウブツ オ モチイタ ジセダイ コツ ナンコツ サイセイ イリョウ ノ ケントウ"}]},"item_8_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2014-06-05"}]},"item_8_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"2011-2013年度科学研究費助成事業（基盤研究（B））研究成果報告書　課題番号：23380185　研究代表者：三角一浩（鹿児島大学・獣医学部・教授）","subitem_description_type":"Other"},{"subitem_description":"皮下脂肪由来間葉系幹細胞（AT-MSC）の凝集塊を積層して円柱の移植構造体を作製した。両側大腿骨の滑車溝に同形・同サイズの骨軟骨欠損部を作り、右に構造体を移植、左は無処置とした。移植後CT検査では、欠損部の周縁と底面から再石灰化が進行し、移植欠損部のX線不透過領域の方が速く縮小した。6か月後の病理組織検査では、移植部は繊維軟骨で、対照部は肉芽組織で充填されていた。12か月後では、移植部で繊維軟骨の一部に硝子軟骨が散見されたが、対照部は表層のみ繊維軟骨であった。移植部深層では軟骨下骨が充実していたが、対照では骨欠損が残存した。人工足場を用いないAT-MSC単独移植による骨軟骨再生が可能となった。","subitem_description_type":"Other"},{"subitem_description":"This study was designed to investigate the regeneration of articular cartilage and subchondral bone using a three-dimensional (3D) construct of adipose tissue-derived mesenchymal stem cells (AT-MSCs). A construct consisting of about 760 spheroids each containing 50,000 autologous AT-MSCs was implanted into an osteochondral defect at the femoral trochlear groove. In CT images after surgery, a radiopaque area emerged from the boundary between the bone and the implant and increased more steadily upward and inward in the implants. Histopathology of the implants after 6 months revealed active endochondral ossification underneath the plump fibrocartilage. Histopathology after 12 months showed that not only was the diminishing fibrocartilage as thick as the surrounding normal cartilage, but massive subchondral bone was also observed. We supposed that implantation of a scaffold-free 3D construct of AT-MSCs will induce regeneration of original structure of cartilage and subchondral bone.","subitem_description_type":"Other"}]},"item_8_full_name_2":{"attribute_name":"著者よみ","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"ミスミ, カズヒロ"}]}]},"item_8_full_name_3":{"attribute_name":"別言語の著者","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"MISUMI, Kazuhiro"}]}]},"item_8_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学"}]},"item_8_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"491","subitem_subject_scheme":"NDC"}]},"item_8_text_24":{"attribute_name":"公開者よみ","attribute_value_mlt":[{"subitem_text_value":"カゴシマ ダイガク"}]},"item_8_text_25":{"attribute_name":"公開者別名","attribute_value_mlt":[{"subitem_text_value":"Kagoshima University"}]},"item_8_text_41":{"attribute_name":"科研費番号 ","attribute_value_mlt":[{"subitem_text_value":"23380185"}]},"item_8_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"三角, 一浩"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-31"}],"displaytype":"detail","filename":"23380185_三角一浩.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"23380185_三角一浩.pdf","url":"https://ir.kagoshima-u.ac.jp/record/11966/files/23380185_三角一浩.pdf"},"version_id":"c7ec8bf3-b279-457c-943d-316c24a35473"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"外科","subitem_subject_scheme":"Other"},{"subitem_subject":"幹細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"再生医療","subitem_subject_scheme":"Other"},{"subitem_subject":"関節","subitem_subject_scheme":"Other"},{"subitem_subject":"骨","subitem_subject_scheme":"Other"},{"subitem_subject":"軟骨","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"骨軟骨欠損モデル動物を用いた次世代骨軟骨再生医療の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"骨軟骨欠損モデル動物を用いた次世代骨軟骨再生医療の検討"}]},"item_type_id":"8","owner":"3","path":["135"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-05-19"},"publish_date":"2015-05-19","publish_status":"0","recid":"11966","relation_version_is_last":true,"title":["骨軟骨欠損モデル動物を用いた次世代骨軟骨再生医療の検討"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-09-01T10:12:51.287287+00:00"}