{"created":"2023-07-25T08:11:44.157879+00:00","id":11977,"links":{},"metadata":{"_buckets":{"deposit":"e2b51d75-bf78-49e1-891c-4e9cb30afb4e"},"_deposit":{"created_by":18,"id":"11977","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"11977"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00011977","sets":["57:80"]},"author_link":["56709"],"item_8_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2014-06-21","subitem_date_issued_type":"Collected"}]},"item_8_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"2011-2013年度科学研究費助成事業（若手研究（B））研究成果報告書　課題番号：23700562　研究代表者：赤坂恵理（鹿児島大学・医歯（薬）学総合研究科・客員研究員）","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"奇形腫から由来する細胞群から歯構成細胞を特異的に非侵襲的に検出するため、エナメル芽細胞特異的な遺伝子の下流に蛍光遺伝子と薬剤耐性遺伝子を繋げた構築体（pT-ARIP）をpiggyBacトランスポゾンによる遺伝子導入システムを用いて導入した。残念ながら、pT-ARIP導入iPS細胞からはそれ自体の奇形腫を得ることができなかった。そこで、遺伝子導入前のiPS細胞から得られた奇形腫を初代培養し、得られた培養細胞に対しRT-PCR解析と免疫組織学的解析を行ったところ、DSPPやAMELXの存在を確認した。以上より、in vitroでiPS細胞から歯形成に係わる細胞群を分化誘導できる可能性が示された。","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"In order to specifically and noninvasively detect a tooth constitution cell among \nteratoma cells, a construct (pT-ARIP), which connected the fluorescent gene and the drug resistance gene that were present downstream from the specific gene in the ameloblast, was introduced via a gene introduction system using the piggyBac transposon. A teratoma was not formed via the introduction of pT-ARIP into  iPS cells. Hence, RT-PCR and immunohistological analyses of the primary cell culture of the teratoma derive \nd from iPS cells was conducted before transgenesis. We confirmed the existence of DSPP and AMELX. As mentioned above, a possibility that the differentiation induction of the cell group concerning tooth formation could be carried out from an iPS cell in vitro was shown.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_8_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"Kagoshima University","subitem_publisher_language":"en"}]},"item_8_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"491","subitem_subject_scheme":"NDC"},{"subitem_subject":"497","subitem_subject_scheme":"NDC"}]},"item_8_text_41":{"attribute_name":"科研費番号 ","attribute_value_mlt":[{"subitem_text_value":"23700562"}]},"item_8_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"赤坂, 恵理","creatorNameLang":"ja"},{"creatorName":"AKASAKA, Eri","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"56709","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000020597562","nameIdentifierScheme":"NRID","nameIdentifierURI":" "}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-31"}],"displaytype":"detail","filename":"23700562_赤坂恵理.pdf","filesize":[{"value":"194.7 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"23700562_赤坂恵理.pdf","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/11977/files/23700562_赤坂恵理.pdf"},"version_id":"ff76b548-16f1-4983-9991-fb7dba46b374"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ES細胞","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"iPS細胞","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"歯形成細胞","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"in vitro組織再構成","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"完全なES/iPS細胞に由来する歯形成細胞誘導に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"完全なES/iPS細胞に由来する歯形成細胞誘導に関する研究","subitem_title_language":"ja"},{"subitem_title":"Induction of tooth constitution cell derived from ES/iPS cells","subitem_title_language":"en"}]},"item_type_id":"8","owner":"18","path":["80"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-05-21"},"publish_date":"2015-05-21","publish_status":"0","recid":"11977","relation_version_is_last":true,"title":["完全なES/iPS細胞に由来する歯形成細胞誘導に関する研究"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-05-24T00:24:47.198517+00:00"}