{"created":"2023-07-25T08:11:44.245142+00:00","id":11979,"links":{},"metadata":{"_buckets":{"deposit":"553c5c37-0260-4095-857d-5974140e0c09"},"_deposit":{"created_by":18,"id":"11979","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"11979"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00011979","sets":["43:81"]},"author_link":["5212"],"item_8_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2014-06-18","subitem_date_issued_type":"Collected"}]},"item_8_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"2011-2013年度科学研究費助成事業（挑戦的萌芽研究）研究成果報告書　課題番号：23658033　研究代表者：久保達也（鹿児島大学・農学部・准教授）","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"カンキツ自家不和合成遺伝子の単離を目的として蕾の発育段階の違いでサブトラクションを行い、花柱特異性によりcitT209を単離した。機能推定の結果、citT209はCu/Zn SODであると推定された。一方、カンキツの蕾にCuSO4処理することによって自家不和合成が打破される可能性を示した。更に、CuSO4により抗酸化酵素であるCu/Zn SOD遺伝子の発現量が高まることが明らかとなり、さらに活性酸素が花粉の発芽および花粉管伸長を抑制することも明らかとなった。蕾へのCuSO4処理により、Cu/Zn SOD遺伝子の発現量が増大し、活性酸素の除去によって不和合成の打破効果が得られるものと推察された。","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"We performed gene expression subtraction of the difference in developmental stages of bud for the purpose of isolation of citrus self-incompatibility gene. As a result,  citT209 was isolated by stylar specificity. Performing the function estimation, it was estimated be a Cu / Zn SOD. On the other hand, self-incompatibility of citrus was shown could be overcome by  CuSO4 sprays to bud. In addition, it is enhanced by  CuSO4 sprays to bud has revealed that the expression level of Cu / Zn SOD gene, which is an antioxidant enzyme. The pollen tube growth and germination of the pollen is inhibited by the active oxygen. Expression level of Cu / Zn SOD gene is increased by spraying  CuSO4 to bud, as a result, was suggested that active oxygen is removed, overcoming the effects of incompatibility can be obtained.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_8_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"Kagoshima University","subitem_publisher_language":"en"}]},"item_8_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"625","subitem_subject_scheme":"NDC"}]},"item_8_text_41":{"attribute_name":"科研費番号 ","attribute_value_mlt":[{"subitem_text_value":"23658033"}]},"item_8_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"久保, 達也","creatorNameLang":"ja"},{"creatorName":"クボ, タツヤ","creatorNameLang":"ja-Kana"},{"creatorName":"KUBO, Tatsuya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5212","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000070359983","nameIdentifierScheme":"NRID","nameIdentifierURI":" "}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-31"}],"displaytype":"detail","filename":"23658033.pdf","filesize":[{"value":"342.9 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"23658033.pdf","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/11979/files/23658033.pdf"},"version_id":"3db46e78-edea-4260-a6cc-a69197194eff"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"カンキツ","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"自家不和合性","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"遺伝子発現特性に基づくカンキツ自家不和合性遺伝子の単離","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"遺伝子発現特性に基づくカンキツ自家不和合性遺伝子の単離","subitem_title_language":"ja"}]},"item_type_id":"8","owner":"18","path":["81"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-05-21"},"publish_date":"2015-05-21","publish_status":"0","recid":"11979","relation_version_is_last":true,"title":["遺伝子発現特性に基づくカンキツ自家不和合性遺伝子の単離"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-04-24T07:02:54.609935+00:00"}