{"created":"2023-07-25T08:11:44.771479+00:00","id":11991,"links":{},"metadata":{"_buckets":{"deposit":"9a6d6ed7-72b7-4189-af3d-bfb8d1c7fadd"},"_deposit":{"created_by":18,"id":"11991","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"11991"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00011991","sets":["57:80"]},"author_link":["56741"],"item_8_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2014-06-27","subitem_date_issued_type":"Collected"}]},"item_8_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"2011-2013年度科学研究費助成事業（基盤研究（C））研究成果報告書　課題番号：23592222　研究代表者：石堂康弘（鹿児島大学・医歯（薬）学総合研究科・准教授）","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"骨粗鬆症は成人の骨の骨形成と骨吸収のバランスが崩れた結果である。シュニュリ3（Shn3）は成熟個体の骨量を負に制御するが、その分子メカニズムについて不明な点も残っている。我々は、Shn3の下流で発現が制御される遺伝子をマイクロアレイによって網羅的に検索し、Alg2という蛋白糖化酵素を同定した。機能を解析すると、これが骨形成に重要なRunx2の転写活性を抑制する事、また軟骨細胞においては、生理的小胞体ストレス関連分子Creb312の発現に重要で、したがって軟骨細胞成熟を正に制御する事も分かった。本研究は骨軟骨組織において、蛋白糖転移酵素が遺伝子発現や転写調節に関わる新しい可能性を示した。","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"Osteoporosis is a result of unbalanced bone formation and resorption in adult bone. Although Schnurri 3 (Shn3) negatively regulates bone volume in adult mice, the underlying molecular mechanism are not fully understood. We searched for genes which expression level were affected by knockdown of Shn3 in osteoblasts by microarray approach, and found that  Alg2 was induced by Shn3.  Alg2 physically interacted with the osteoblast differentiation master regulator Runx2 to interfere its nuclear localization, which resulted in its suppressed transcriptional activity. In chondrocytes,  Alg2 induced  Creb3l2, which is a crucial gene in the important physiological endoplasmic reticulum stress during chondrogenesis, to support chondrocyte maturation. Our findings revealed a novel possibility that mannosyltransferase genes play crucial roles in gene transcription and expression in cells of bone and cartilage.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_8_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"Kagoshima University","subitem_publisher_language":"en"}]},"item_8_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"493","subitem_subject_scheme":"NDC"}]},"item_8_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"石堂, 康弘","creatorNameLang":"ja"},{"creatorName":"ISHIDOU, Yasuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-31"}],"displaytype":"detail","filename":"23592222_石堂康弘.pdf","filesize":[{"value":"164.5 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"23592222_石堂康弘.pdf","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/11991/files/23592222_石堂康弘.pdf"},"version_id":"1da57025-d147-4065-a1cb-020efe8da731"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Shn3","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Hivep3","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Alg2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"骨芽細胞分化","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"軟骨細胞分化","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"Runx2","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"骨量規定転写因子シュニュリ3の新規標的遺伝子同定と機能解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"骨量規定転写因子シュニュリ3の新規標的遺伝子同定と機能解析","subitem_title_language":"ja"},{"subitem_title":"Functional analysis of the novel target gene of bone volume-regulating transcripton factor Schnurri-3.","subitem_title_language":"en"}]},"item_type_id":"8","owner":"18","path":["80"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-05-22"},"publish_date":"2015-05-22","publish_status":"0","recid":"11991","relation_version_is_last":true,"title":["骨量規定転写因子シュニュリ3の新規標的遺伝子同定と機能解析"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-05-24T00:24:43.037727+00:00"}