@techreport{oai:ir.kagoshima-u.ac.jp:00011992,
 author = {小宮, 節郎},
 month = {2016-10-31},
 note = {2011-2013年度科学研究費助成事業（基盤研究（C））研究成果報告書　課題番号：23592195　研究代表者：小宮節郎（鹿児島大学・医歯（薬）学総合研究科・教授）, 骨軟部肉腫の発生・増殖におけるGOLPH3とそのアイソフォームであるGOLPH3Lの機能の解析を行った。横紋筋肉腫の患者腫瘍組織におけるGOLPH3/GOLPH3Lの発現を検討したところ正常筋肉組織と比較して横紋筋肉腫組織ではGOLPH3/GOLPH3L分子が発現亢進していた。また横紋筋肉腫の細胞株でも正常筋芽細胞と比較してGOLPH3/GOLPH3L分子が発現亢進していた。GOLPH3/GOLPH3Lの横紋筋肉腫における機能解析のためにGOLPH3/GOLPH3LをRNAiを用いて選択的にノックダウンすると横紋筋肉腫細胞の増殖が抑制された。, GOLPH3 was originally identified by proteomic analyses of Golgi proteins localized  in the trans-Golgi network. Recently, it was reported that GOLPH3 is up-regulated in various types of malignancies. In order to explore the function of GOLPH3 and its isoform, GOLPH3L, in the pathogenesis of rhabdomyosarcoma, we investigated the expression and knockdown effects of GOLPH3/GOLPH3L in human rhabdomyosarcoma. Western blot analysis and real-time PCR revealed that human rhabdomyosarcoma cell lines and biopsyspecimens exhibited an increased expression of GOLPH3/GOLPH3L. GOLPH3/GOLPH3L knockdown by  siRNA prevented the proliferation of human rhabdomyosarcoma cell lines. In addition, double-knockdown of GOLPH3/GOLPH3L also prevented the proliferation of rhabdomyosarcoma cell lines. Our findings improve the understanding of 
rhabdomyosarcoma pathogenesis and suggest that the knockdown of GOLPH3/GOLPH3L may be an effective treatment for rhabdomyosarcoma.},
 title = {GOLPH3関連遺伝による骨軟部腫瘍治療研究},
 year = {}
}