{"created":"2023-07-25T08:11:44.815164+00:00","id":11992,"links":{},"metadata":{"_buckets":{"deposit":"1213b786-0142-4976-8795-92c5a6d6352c"},"_deposit":{"created_by":3,"id":"11992","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"11992"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00011992","sets":[]},"author_link":["118112","22679","118113"],"item_8_alternative_title_18":{"attribute_name":"別言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Role of GOLPH3 and GOLPH3L in the proliferation of human rhadomyosarcoma"}]},"item_8_alternative_title_19":{"attribute_name":"タイトルよみ","attribute_value_mlt":[{"subitem_alternative_title":"GOLPH3 カンレン イデンシ ニヨル コツ ナンブ シュヨウ チリョウ ケンキュウ"}]},"item_8_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2014-06-30"}]},"item_8_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"2011-2013年度科学研究費助成事業（基盤研究（C））研究成果報告書　課題番号：23592195　研究代表者：小宮節郎（鹿児島大学・医歯（薬）学総合研究科・教授）","subitem_description_type":"Other"},{"subitem_description":"骨軟部肉腫の発生・増殖におけるGOLPH3とそのアイソフォームであるGOLPH3Lの機能の解析を行った。横紋筋肉腫の患者腫瘍組織におけるGOLPH3/GOLPH3Lの発現を検討したところ正常筋肉組織と比較して横紋筋肉腫組織ではGOLPH3/GOLPH3L分子が発現亢進していた。また横紋筋肉腫の細胞株でも正常筋芽細胞と比較してGOLPH3/GOLPH3L分子が発現亢進していた。GOLPH3/GOLPH3Lの横紋筋肉腫における機能解析のためにGOLPH3/GOLPH3LをRNAiを用いて選択的にノックダウンすると横紋筋肉腫細胞の増殖が抑制された。","subitem_description_type":"Other"},{"subitem_description":"GOLPH3 was originally identified by proteomic analyses of Golgi proteins localized  in the trans-Golgi network. Recently, it was reported that GOLPH3 is up-regulated in various types of malignancies. In order to explore the function of GOLPH3 and its isoform, GOLPH3L, in the pathogenesis of rhabdomyosarcoma, we investigated the expression and knockdown effects of GOLPH3/GOLPH3L in human rhabdomyosarcoma. Western blot analysis and real-time PCR revealed that human rhabdomyosarcoma cell lines and biopsyspecimens exhibited an increased expression of GOLPH3/GOLPH3L. GOLPH3/GOLPH3L knockdown by  siRNA prevented the proliferation of human rhabdomyosarcoma cell lines. In addition, double-knockdown of GOLPH3/GOLPH3L also prevented the proliferation of rhabdomyosarcoma cell lines. Our findings improve the understanding of \nrhabdomyosarcoma pathogenesis and suggest that the knockdown of GOLPH3/GOLPH3L may be an effective treatment for rhabdomyosarcoma.","subitem_description_type":"Other"}]},"item_8_full_name_2":{"attribute_name":"著者よみ","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"コミヤ, セツロウ"}]}]},"item_8_full_name_3":{"attribute_name":"別言語の著者","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"KOMIYA, Setsuro"}]}]},"item_8_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学"}]},"item_8_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"493","subitem_subject_scheme":"NDC"}]},"item_8_text_24":{"attribute_name":"公開者よみ","attribute_value_mlt":[{"subitem_text_value":"カゴシマ ダイガク"}]},"item_8_text_25":{"attribute_name":"公開者別名","attribute_value_mlt":[{"subitem_text_value":"Kagoshima University"}]},"item_8_text_41":{"attribute_name":"科研費番号 ","attribute_value_mlt":[{"subitem_text_value":"23592195"}]},"item_8_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小宮, 節郎"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-31"}],"displaytype":"detail","filename":"23592195_小宮節郎.pdf","filesize":[{"value":"336.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"23592195_小宮節郎.pdf","url":"https://ir.kagoshima-u.ac.jp/record/11992/files/23592195_小宮節郎.pdf"},"version_id":"74c86f71-b241-4b00-b197-41de5105d0fa"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"横紋筋肉腫","subitem_subject_scheme":"Other"},{"subitem_subject":"GOLPH3","subitem_subject_scheme":"Other"},{"subitem_subject":"GOLPH3L","subitem_subject_scheme":"Other"},{"subitem_subject":"外科系臨床医学・整形外科学","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"GOLPH3関連遺伝による骨軟部腫瘍治療研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"GOLPH3関連遺伝による骨軟部腫瘍治療研究"}]},"item_type_id":"8","owner":"3","path":["80"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-05-22"},"publish_date":"2015-05-22","publish_status":"0","recid":"11992","relation_version_is_last":true,"title":["GOLPH3関連遺伝による骨軟部腫瘍治療研究"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-12-25T07:18:47.705213+00:00"}