@article{oai:ir.kagoshima-u.ac.jp:00012251,
 author = {Shiozaki, Yasushi and Taoka, Yousuke and Yoshikawa, Takeshi and Sakata, Taizo},
 journal = {鹿児島大学水産学部紀要=Memoirs of Faculty of Fisheries Kagoshima University},
 month = {2016-10-31},
 note = {Bacillus sp., PU-T8 strain, isolated from tiger puffer fish Takijugu rubripes showed growth-inhibitory activity against bacterial fish  pathogens such as Edwardsiella tarda and Vibrio anguillarum. Physiological characterization and the phylogenetic analysis based on  16SrDNA sequences indicated that the strain PU-T8 is most closely related to Bacillus subtilis strains. The strain was considerably  stable in acidic buffers (pH 2.0-4.0) and in buffers with digestive enzymes such as pepsin (pH 2.0) and pancreatin (pH 8.0). Cells suspended in PBS also remainded viable at 103 cfu/ml after heat treatment at 100ｰC for 60 min. The strain PU-T8 grew well in Z-AII  broth media with 0.1 M to 2.0 M NaCl. Crude extract obtained with ethyl acetate from the culture supernatant of the strain PU-T8 showed growth-inhibitory activity against fish pathogens on the double-layer agar plates.},
 pages = {79--86},
 title = {Characterization of Bacillus sp. PU-T8 Isolated from Tiger Puffer Fish Takifugu rubripes as Probiotics},
 volume = {55},
 year = {}
}