@article{oai:ir.kagoshima-u.ac.jp:00012360,
 author = {AMROZI, AMROZI and KAMIMURA, Shunichi and ANDO, Takaaki and HAMANA, Katsumi},
 journal = {Memoirs of the Faculty of Agriculture, Kagoshima University},
 month = {2016-10-31},
 note = {The aims were to determine the turnover of the first follicular wave in Japanese black cows and to quantify immunohistological characteristics of identified DF and SF identified in vivo and extracted by means of ovariectomy on Day 7 and Day 10 (Day 0 = day of estrus). Six cases of the first follicular wave in Japanese black cows were observed using ultrasonic detection. The number of follicles, diameter of DF and SF, and prevalence of apoptotic cells m the membrana granulosa and theca calls were studied. Injection of 25 mg PGF_<2α> induced ovulation 90 ± 19 h later. At the follicular wave emergence, 13.5 ± 9.5 (mean ± SD) follicles of 2 - 5 mm in diameter were found. Retrospectively, future dominant follicle and largest subordinate follicle were observed 4.9 mm in diameter for the first appearance. Deviation of the DF and SF occurred on Day 3 pm (n = 6) with mean diameters of 8.9 ± 1.3 mm and 6.8 ± 0.9 mm, respectively. DF developed until day 8 reaching a maximum diameter 14.6 ± 2.1 mm (n = 3) and then regressed. The follicular wall of DF showed characteristics in keeping with healthy follicles on Day 7, becoming slightly atretic in DF on Day 10, while SF showed heavy atresia both on Day 7 and Day 10 under hematoxyhn and eosm staining. As to the prevalence of apoptotic cells, DF showed 3.1 ± 2.7% and 20.9 ± 0.6% on Day 7 and on Day 10 respectively in membrane granulosa, and 2.5 ± 2.8% and 6.5 ± 1.7% on Day 7 and on Day 10 respectively in theca cells. In conclusion, these results showed that: 1) the first follicular wave in cows is characterized by one day recruitment of small follicles and a gradual divergence of growth rates in the future dominant and largest subordinate follicles; 2) early regression of the DF is preceded by severe apoptosis.},
 pages = {45--53},
 title = {Histoendocrinological Observation of Dominant Follicles and Subordinate Follicles in First Follicular Wave in Japanese Black Cows},
 volume = {38},
 year = {}
}