{"created":"2023-07-25T08:12:22.985830+00:00","id":12713,"links":{},"metadata":{"_buckets":{"deposit":"c5274bf7-154a-4adf-9d4b-508242d960c9"},"_deposit":{"created_by":18,"id":"12713","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"12713"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00012713","sets":["43:81"]},"author_link":["59788"],"item_8_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2011-03-31","subitem_date_issued_type":"Collected"}]},"item_8_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"2007-2010年度科学研究費補助金(基盤研究(A))研究成果報告書 課題番号：19208026　研究代表者：藤崎幸藏 (鹿児島大学農学部教授)","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"1. マダニによって人獣に媒介されるBabesia原虫のマダニ体内における生殖期・スポロゴニー期の虫体(ガメートからスポロゾイトに至る発育期の虫体)と顕著に反応・結合するマダニ生物活性物質(TBM)として、マダニの卵黄蛋白前駆体(Vg)とその受容体のVgR、ならびに血液消化酵素(システインプロテアーゼ)のlongipain を発見した。2. VgとVgRは、RNA干渉法(RNAi)によって原虫媒介を完全に阻止し、抗体のワクチン効果も多大であったことから、現在、これらと結合する原虫分子の同定などの、Babesia媒介阻止ワクチンとしての応用に必要な研究を鋭意実施中である。3. しかし、longipainは、RNAiによる遺伝子ノックダウンによって、B. gibsoni原虫の増殖を約3倍程度に増大させるものであり、遺伝子のover-expression(過剰発現)系が未開発の現状では、媒介阻止ワクチンして応用することは難し\nい現状にある。4. 一方、Babesiaに対する顕著な発育・増殖の阻止効果を有するTBMの探索を行う中で、細胞内寄生性のBabesiaの必須栄養源であるマダニ細胞内のバイオマスの動態に関わるTBMに興味を持ち、5. マダニの細胞内蛋白の転写、翻訳開始、翻訳後修飾、小胞体・ゴルジ間輸送に関与する一連のTBMに加えて、細胞内蛋白質のバルク分解機構であるオートファジー(AT)に関連するTBM多数を同定・特性解明して、いずれも世界に先駆けて報告した。6. これらの研究を通じて、アミノ酸シグナルによって細胞内タンパク質の動態を中枢的に制御しているTORカスケード(TORp)が、マダニの生存とマダニ細胞内の寄生原虫の増殖に果たす重要性の大きさが明らかになり、7. TORp構成分子を媒介阻止ワクチンの候補分子として活用しうる可能性が示されてきた。","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"1. We could identify for the first time vitellogenins (Vg), vitellogeni-receptor (VgR), and longipain as tick-bioactive molecules (TBM) specifically reacted with sexual and sporogonic stages (S-S satages) of Babesia parasites developing in the vector ticks. 2. Since Vg and VgR could inhibit completely the Babesia transmission in ticks treated with their dsRNA and could suppress effectively the Babesia transmission in animals vaccinated with them, the identification of Babesia molecules reacted with them is now concentrated. 3. However, longipain could promote Babesia development in vector ticks after RNAi, it is considered that its application as candidate molecule for the transmission-blocking vaccine may be difficult under the current research situation lacking of the TBM over0expression systems. 4. During the period of this research, we started to focus on TBMs involved in cellular biomass (CB)control in host cells for Babesia parasites because it was noticed that the development of intra-cellular parasites is fundamentally influenced by the essential nutrients supply under the control of CB. 5. Therefore, the molecular identification and characterization of a series of TBMs involving in transcription, translation, post translational modification, and vesicular transport, autophagy (AT) of intra-cellular proteins are concentrated for the first time. 6. These studies are elucidating the significance of TOR pathways (TORs) as candidate molecules for the transmission-blocking vaccine.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_8_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"Kagoshima University","subitem_publisher_language":"en"}]},"item_8_relation_32":{"attribute_name":"関連（relation）","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://kaken.nii.ac.jp/ja/p/19208026","subitem_relation_type_select":"URI"}}]},"item_8_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"649","subitem_subject_scheme":"NDC"}]},"item_8_text_41":{"attribute_name":"科研費番号 ","attribute_value_mlt":[{"subitem_text_value":"19208026"}]},"item_8_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"藤崎, 幸蔵","creatorNameLang":"ja"},{"creatorName":"フジサキ, コウゾウ","creatorNameLang":"ja-Kana"},{"creatorName":"FUJISAKI, Kozo","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-31"}],"displaytype":"detail","filename":"19208026seika.pdf","filesize":[{"value":"241.1 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"19208026seika.pdf","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/12713/files/19208026seika.pdf"},"version_id":"a17eb3c6-d549-46d0-b968-01b4e8cc6f14"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"マダニ","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"Babesia原虫","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"マダニ生物活性分子","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"TBM","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"アミノ酸","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"TOR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"オートファジー","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"ワクチン","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"マダニ媒介原虫病のTRANSMISSION-BLOCKING VACCINE開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"マダニ媒介原虫病のTRANSMISSION-BLOCKING VACCINE開発","subitem_title_language":"ja"},{"subitem_title":"Developments of transmission-blocking vaccine for tick-borne protozoan diseases","subitem_title_language":"en"}]},"item_type_id":"8","owner":"18","path":["81"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-01-06"},"publish_date":"2015-01-06","publish_status":"0","recid":"12713","relation_version_is_last":true,"title":["マダニ媒介原虫病のTRANSMISSION-BLOCKING VACCINE開発"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-04-24T07:02:52.768374+00:00"}