@article{oai:ir.kagoshima-u.ac.jp:00012757, author = {SAKATA, Taizo and UEDA, Kazuaki and KAKIMOTO, Daiichi}, journal = {鹿児島大学水産学部紀要=Memoirs of Faculty of Fisheries Kagoshima University}, month = {2016-10-31}, note = {Extracellular ptotease(s) of a marine Pseudomonas sp. I-6 was produced dependent of polypeptone concentration of the suspending medium but independent of yeast extract in the presence of polypeptone and artificial sea water (ASW). In a basal suspension medium containing polypeptone (0.5%), yeast extract (0.1%) and NaCl (3.0%), protease formation of marine isolates did not occur during 6 hr incubation. The addition of Ca^++ ion to the basal medium stimulated protease formation but Mg^++ ion did not replace Ca^++ ion. In the experiment of washed cell suspensions, K^+ ion was necessary for protease formation of both marine isolates and Bacillus subtilis. The effect of K^+ ion was almost identical with that of yeast extract on protease formation. On the other hand, the supplement of Ca^++ ion was indispensable to marine isolates but not to B. subtilis except as a trace ion contaminant.}, pages = {63--69}, title = {Studies on the Proteases of Marine Bacteria II : Effect of Cations on Protease Formation}, volume = {26}, year = {} }