{"created":"2023-07-25T08:12:34.833358+00:00","id":12959,"links":{},"metadata":{"_buckets":{"deposit":"8416a8c9-8a0c-43fc-a2e7-586a877c981d"},"_deposit":{"created_by":29,"id":"12959","owners":[29],"pid":{"revision_id":0,"type":"depid","value":"12959"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00012959","sets":["228:280:4982","41:42"]},"author_link":["65251"],"item_7_biblio_info_5":{"attribute_name":"収録雑誌名","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1961-01-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"150","bibliographicPageStart":"82","bibliographicVolumeNumber":"13","bibliographic_titles":[{"bibliographic_title":"鹿児島大学教育学部研究紀要. 自然科学編","bibliographic_titleLang":"ja"},{"bibliographic_title":"Bulletin of the Faculty of Education, Kagoshima University. Natural science","bibliographic_titleLang":"en"}]}]},"item_7_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"1961-01-01","subitem_date_issued_type":"Issued"}]},"item_7_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Part 1 : \nImmunoserological Studies on that Change of serum protein which follows the growth of Cock","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"Studies on the relations between such phenomena as the growth and reproduction\nof cock and the change of serum protein were performed chiefly immunoserolo\ngically from the viewpoint of metabolism of endogenous hormone.\nThe results obtained are summarized as follows :\nIn the first chapter, the change of serum protein following the growth of cock\nwas investigated immunoserologically. And the following facts were revealed; in\nMCS (1-day-old male chick serum) there is a specific protein fraction termed Fraction\nM that cannot be noticed in GCS (growing male chick serum) and ACS (adult cock\nserum), and the fraction continues to be recognized till it is about two weeks after\nhatching; in GCS and ACS there is another specific protein fraction termed Fraction\nA which cannot be noticed in MCS, and it appears during a period from about two\nto three weeks after hatching and afterwards it can be detected till it becomes an\nadult cock.\nFurther the following facts were found as a result of agar gel precipitin ring\ntest; there are immunoserologically at least three kinds of fraction in cock serum\nthrough the whole career from hatching till it becomes an adult cock; and there\nare in each stage specific protein fractions such as Fraction M and Fraction A,\nconsequently from these facts the construction of serum proteins of MCS and ACS\nwas supposed as shown in Fig. 11.\nNo immunoserological specificity can be noticed between GCS and ACS.\nOn one hand, each cock serum of various ages was electrophoretically investigated,\nbut the existence of the specific protein fractions mentioned above could not\nbe made clear.","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"In the second chapter, the serological nature of Fraction M and Fraction A was investigated.\nIn order to examine the serological nature of the above mentioned specific\nprotein fractions (M and A), anti-LHS (laying hen serum) and anti-CSS (cock\nsemen serum) were prepared, and ACS, MCS, egg yolk Extracts, Albumin, LHS,\nCSS were used as antigenic substance.\nThe results obtained are summarized as follows :\nJudging from the fact that the migration velocity of precipitin band in agar\ngel precipitin ring test shows linear regression, both in anti-MCS and anti-ACS, in\nconformity with migration formula, the chemical identification of each Fraction\nseemed possible. From the fact that Fraction M showed positive reaction to egg yolk\nextracts and LHS, it was presumed that Fraction M was very closely related to egg\nyolk extracts, and the fact made it clear that MCS, egg yolk and LHS have common\nprotein fraction.\nOn the other hand, it was found that Fraction A had nothing to do with albumin\nand MCS, showing positive reaction to CSS and LHS, and that reaction pattern was\nso similar that both ACS and CSS have the same construction of proteins.\nIt was made clear from the above description that LHS holds Fraction M and\nFraction A in common, then LHS was considered to be a union of both protein\nfractions of ACS and MCS, but the result of a later experiment caused the presumption\nto be modified and had it considered that LHS has a component (x~x') besides\n(MCS+ACS). It is considered that Fraction M or Fraction M+ (x~x') plays a pretty\nimportant part in the construction of LHS proteins, and that a comparatively small\nproportion is occupied by Fraction A.","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"It is confirmed that, from the result of reaction by anti-CSS, ACS and CSS shows\na protein construction very similar to each other, and further that Fraction M has nothing to do with CSS.\nFrom these facts, it was presumed that Fraction M is closely related to egg\nyolk vitellin, and these facts showing that Fraction A is noticed in nothing but\nsemen serum, ACS, LHS and so forth, led the author to presume that the Fraction\nA is related to a substance produced by Androgen.\nIn the third chapter, therefore, the effects caused by sexual hormone injection,\nthe removal of yolk sac, problems concerning the immunoserological specificity of\nserum of male chick dieted with much yolk and the physiological roles played by\nFraction M in the earlier stage of chick were investigated. The results are summarized\nas follows :\nWhen Estrogen is injected into chicks and adult cocks, substance corresponding\nto Fraction M appears; when Androgen is injected into male day old chicks, substance\ncorresponding to Fraction A appears; when yolk sac is removed, Fraction M\nmakes regress and inversely, a surprisingly great quantity of Fraction M was\nnoticed in the serum of chick dieted with much yolk.\nThis experiment raised several problems concerning the effect of hormone. That is to say, when Estrogen is injected into the cock, Fraction M is supposed to have\nsynergism and Fraction A has antagonism to Estrogen. When a much dose of\nAndrogen is injected into the male day old chick, it is observed that its comb increases\nremarkably in weight a week after the injection compared with that at a normal stage,\nand that the testes, on the other hand, becomes atrophied. In this case, no substance\ncorresponding to Fraction M is noticed in the serum, while some substance corresponding to Fraction A is noticed. This fact is a reverse phenomena to a normal\nchick serum in a case where no treatment is given, which is a very important fact.\nThe point to be debated here is a serological difference between LHS and MCS.","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"It was previously mentioned that the construction of protein of laying hen serum\nwas supposed to be ACS+MCS+(x~x'). Thereupon it is a very interesting point\nto know in what position and form and proportion the Fraction M exists in the\nprotein fraction of laying hen.\nThe following are the summarized results obtained from serum reaction : Antigenic substances showing positive reaction to Fraction M are MCS, LHS,\negg yolk extracts, estrogen-treated Cock serum, serum of male chick dieted with\nmuch yolk and so forth, while antigenic substances showing positive reaction to\nanti-LHS are MCS, ACS and LHS. When the anti-LHS is absorbed by MCS, LHS\nproved positive fraction; when the anti-LHS is absorbed by ACS, both MCS and\nLHS proved positive fraction.\nFrom these reactions, it is considered that a comparatively small proportion is\noccupied by protein fraction proper to ACS in LHS. When the anti-LHS is absorbed\nby MCS, Will it not be considered that ACS fraction is removed accompanyingly?\nThis point is left unsolved.\nIf the main constituent of serum protein proper to laying hen is protein complex\nof Lipoprotein, it is considered that it may be contained in (x~x'). It is a\nquestion to find where is a substance appearing correspondingly to Androgen injection,\nand probably it seems proper to think that it is contained in Fraction A itself.\nIn any case, the (x~x') component is considered to be the main component of\na specific protein of LHS, and it will be necessary that the relation between this\ncomponent and MCS, and between Fraction A and Androgen will be fundamentally\ninvestigated.\nFrom the finding that anti-MCS injection impedes the growth of chick of 1~2\nweeks old in a certain degree, it is confirmed that Fraction M plays a physiological role\nin an earlier growth of chick. But it has not been elucidated by the author in detail\nwhether it is so important protein fraction as to control the growth of chick.","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"Part 2 : Immunoserological Studies on the Hypophysial Gonadotropin of Cock","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"The result obtained by investigating the change of serum protein that accompanies the growth of cock from the viewpoint of metabolism of endogenous hormone\nhas already been mentioned in Part I.\nThere it was reported that the phase of growth was divided into three stages\nand using the serum at each stage as antigen, the specific serological difference\nbetween the stages was elucidated and that the protein fraction proper to each\nstage was deeply related to Estrogen or Androgen. The existence of pituitary growth\nhormone as a great element having a share in the growth of an animal cannot be\nignored. As the existence of growth hormone in the cock was not made clear, the\nlevel of GTH (Gonado-trophic hormone) in blood was going to be immunoserologically investigated fundamentally.\nNow, as whether the gonadotrophic substance of cock pituitary gland had\nantigenicity or not had not been fundamentally elucidated, the author has mad up his mind to examine that point.\nAs it is said that there is much to be debated whether there is anti-hormone\nformation for protein hormone, the problem was considered bibliographically in the\nfirst chapter and it was pointed out that the formation of antihormone was inclined\nto be affirmed. In the second chapter, it was reported that anti-hormone formation for cock hypophysial gonadotrophic hormone had been affirmed by means of bio-assay. The\ncock anterior pituitary acetone dried powder which had been suspended in oil adjuvant was used as antigen. When a cock was injected with 1~2cc. of antiserum at\nthe same time with anterior-pituitary acetone powdered preparation, it was noticed\nthat 40~50% of gonadotrophic activity was inhibited in any case and that antiserum\nwas higher in its inhibitory action in a case of 2cc. injection than 1cc.","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"When a cock was used as an animal for immunization or when antigenserum\nwas not suspended in oil Adjuvant, such antihormone formation could not be proved.\nThe author attempted an immunoserological assay of hormone contained in blood\nwith antihormone as an index.\nFrom the necessity of measuring antibody titer in the process of immunization,\nthe anterior pituitary powder which was dissolved in MCS was used as antigenic\nliquid, and immunoassay was attempted by means of the technique of precipitin ring\ntest and agar gel precipitin test, by using immune rabbit serum obtained.\nThe result showed, as reported in the third chapter, that a certain degree of\nspecific reaction could be noticed, and the immunoassay of cock gonadotrophic\nhormone was considered to be difficult for the time being. But immunoassay for\nGTH is thought possible if any specific substance could be removed from anti-CHG\nrabbit serum and if such conditions could be fulfilled as the constancy of anti GTH\nactivity in antiserum and the raising the purity of the fractionation of gonadotrophic\nsubstance.\nOn the other hand, it was generally accepted that frequent injection of protein\nhormone would produce an antibody, namely, antihormone, which fact was confirmed by the author in his experiment. The fact has been one of the greatest obstacles\nin injecting protein hormone for test or for the object of curing. Therefore, when\nseveral kinds of investigation were made as to the method of neutralizing the antihormone,\nit was made clear by treating with serum in an adjuvant manner that the\nanti-serum containing antihormone has proper antigenicity.\nThe results obtained by experiment in twice and some discussions concerning\nthem were reported in the fourth chapter.\nWhat is to say, when the inhibition of antihormone and degree of neutralization\nwere expressed by the percentage of neutralization, the titer was found to be 50~60% or so.\nIf some method of increasing the amount of anti-antiserum injected and heightening\nthe antibody titer is to be employed, it seems that a more effective inhibition\nof antihormone and neutralizing action will appear, and the method will have a very\nimportant meaning in the way of clinical application and thereby it is thought\npossible to remove one of the obstacles to application of protein hormone.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_7_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"Kagoshima University","subitem_publisher_language":"en"}]},"item_7_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03896692","subitem_source_identifier_type":"PISSN"}]},"item_7_source_id_9":{"attribute_name":"NII書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00040261","subitem_source_identifier_type":"NCID"}]},"item_7_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"481","subitem_subject_scheme":"NDC"}]},"item_7_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"田代, 一男","creatorNameLang":"ja"},{"creatorName":"TASHIRO, Kazuo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"65251","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-31"}],"displaytype":"detail","fileDate":[{"fileDateType":"Issued","fileDateValue":"1961-01-01"}],"filename":"AN00040261_v13_p82-150.pdf","filesize":[{"value":"68.7 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"AN00040261_v13_p82-150.pdf","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/12959/files/AN00040261_v13_p82-150.pdf"},"version_id":"0cdee35f-8d70-454a-a03e-614f726058c8"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"雄鶏の血清蛋白質に関する免疫血清学的研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"雄鶏の血清蛋白質に関する免疫血清学的研究","subitem_title_language":"ja"},{"subitem_title":"Immunoserological Studies on the Cock Serum Protein","subitem_title_language":"en"}]},"item_type_id":"7","owner":"29","path":["42","4982"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-06-24"},"publish_date":"2015-06-24","publish_status":"0","recid":"12959","relation_version_is_last":true,"title":["雄鶏の血清蛋白質に関する免疫血清学的研究"],"weko_creator_id":"29","weko_shared_id":-1},"updated":"2024-12-16T07:09:31.215160+00:00"}