{"created":"2023-07-25T08:12:42.365114+00:00","id":13106,"links":{},"metadata":{"_buckets":{"deposit":"e5d7470e-51f6-40e2-b521-2e47169271f2"},"_deposit":{"created_by":18,"id":"13106","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"13106"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00013106","sets":["57:78"]},"author_link":["117712","117713","117714","117715","117716","117717","117718","117719","117720"],"item_6_biblio_info_5":{"attribute_name":"収録雑誌名","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"906","bibliographicPageStart":"899","bibliographicVolumeNumber":"39","bibliographic_titles":[{"bibliographic_title":"International Journal of Oncology","bibliographic_titleLang":"en"}]}]},"item_6_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2011-06-14","subitem_date_issued_type":"Issued"}]},"item_6_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"The Hedgehog pathway functions as an organizer in embryonic development. Recent studies have shown that mutation of the PTCH1 gene involved in the Hedgehog path-way affects rhabdomyosarcoma development. However, the expression of Hedgehog pathway molecules in human rhabdomyosarcoma cells has not been well clarified. In addition, the effect of pharmacological inhibition of the Hedgehog pathway is not known. We investigated the expression of the genes involved in the Hedgehog pathway using human rhabdomyosarcoma cell lines and biopsy specimens. Further, we evaluated the effect of pharmacological inhibition of the Hedgehog pathway using cyclopamine or GANT61 by WST assay, cell proliferation assay and cell death detection assay. Real-time PCR revealed that human rhabdomyosarcoma cell lines and biopsy specimens overexpressed the following genes: Sonic hedgehog, Indian hedgehog, Desert hedgehog, PTCH1, SMO, GLI1, GLI2 and ULK3. Immunohistochemistry revealed that rhabdomyosarcoma cell lines and biopsy specimens expressed SMO and GLI2. Inhibition of SMO by cyclopamine slowed the growth of human rhabdomyosarcoma cell lines. Similarly, inhibition of GLI by GANT61 slowed the growth of human rhabdomyosarcoma cell lines. Inhibition of cell proliferation and apoptotic cell death together prevented the growth of rhabdomyosarcoma cells by cyclopamine and GANT61 treatment. Our findings suggest that pharmacological inhibition of the Hedgehog pathway may be a useful approach for treating rhabdomyosarcoma patients.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_6_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"Spandidos Publications","subitem_publisher_language":"en"}]},"item_6_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3892/ijo.2011.1076","subitem_relation_type_select":"DOI"}}]},"item_6_relation_32":{"attribute_name":"関連（relation）","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.spandidos-publications.com/ijo/39/4/899","subitem_relation_type_select":"URI"}}]},"item_6_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Spandidos Publications","subitem_rights_language":"en"}]},"item_6_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"10196439","subitem_source_identifier_type":"PISSN"}]},"item_6_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"494.5","subitem_subject_scheme":"NDC"}]},"item_6_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kawabata, Naoya","creatorNameLang":"en"},{"creatorName":"カワバタ, ナオヤ","creatorNameLang":"ja-Kana"},{"creatorName":"川畑, 直也","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ijiri, Kosei","creatorNameLang":"en"},{"creatorName":"イジリ, コウセイ","creatorNameLang":"ja-Kana"},{"creatorName":"井尻, 幸成","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishidou, Yasuhiro","creatorNameLang":"en"},{"creatorName":"イシドウ, ヤスヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"石堂, 康弘","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamamoto, Takuya","creatorNameLang":"en"},{"creatorName":"ヤマモト, タクヤ","creatorNameLang":"ja-Kana"},{"creatorName":"山元, 拓哉","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagao, Hiroko","creatorNameLang":"en"},{"creatorName":"ナガオ, ヒロコ","creatorNameLang":"ja-Kana"},{"creatorName":"永尾, 宗子","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagano, Satoshi","creatorNameLang":"en"},{"creatorName":"ナガノ, サトシ","creatorNameLang":"ja-Kana"},{"creatorName":"永野, 聡","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Maeda, Shingo","creatorNameLang":"en"},{"creatorName":"マエダ, シンゴ","creatorNameLang":"ja-Kana"},{"creatorName":"前田, 真吾","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Komiya, Setsuro","creatorNameLang":"en"},{"creatorName":"コミヤ, セツロウ","creatorNameLang":"ja-Kana"},{"creatorName":"小宮, 節郎","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Setoguchi, Takao","creatorNameLang":"en"},{"creatorName":"セトグチ, タカオ","creatorNameLang":"ja-Kana"},{"creatorName":"瀬戸口, 啓夫","creatorNameLang":"ja"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-31"}],"displaytype":"detail","filename":"Pharmacological inhibition of the Hedgehog pathway prevents human rhabdomyosarcoma cell growth.pdf","filesize":[{"value":"666.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Pharmacological inhibition of the Hedgehog pathway prevents human rhabdomyosarcoma cell growth.pdf","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/13106/files/Pharmacological inhibition of the Hedgehog pathway prevents human rhabdomyosarcoma cell growth.pdf"},"version_id":"075aaa61-d893-42e3-9d92-319b4c8b2daa"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"rhabdomyosarcoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Hedgehog","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cyclopamine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"GANT61","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal 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