{"created":"2023-07-25T08:12:48.705967+00:00","id":13245,"links":{},"metadata":{"_buckets":{"deposit":"17b54a7b-aaab-43ed-9a0e-1719e3d2cb6b"},"_deposit":{"created_by":3,"id":"13245","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"13245"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00013245","sets":[]},"author_link":["118129","14877","118128"],"item_8_alternative_title_18":{"attribute_name":"別言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"A study of osteoblast differentiation and activation in Msx2 gene-transfer ES/iPS cells."}]},"item_8_alternative_title_19":{"attribute_name":"タイトルよみ","attribute_value_mlt":[{"subitem_alternative_title":"ES/iPS サイボウ エノ Msx2 イデンシ ドウニュウゴ ノ コツガ サイボウ エノ ブンカ・ キノウ カッセイ ニカンスル ケンキュウ"}]},"item_8_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2014-06-17"}]},"item_8_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"2011-2013年度科学研究費助成事業（若手研究（B））研究成果報告書　課題番号：23792438　研究代表者：山本芳丈（鹿児島大学・医歯（薬）学総合研究科・助教）","subitem_description_type":"Other"},{"subitem_description":"歯科矯正治療において不可欠である骨組織のリモデリングや再生を担う骨芽細胞の分化制御機構に関与すると考えられているMsx2遺伝子の役割については、これまで殆んど明らかにされていない。そこで、本研究ではマウスES細胞とiPS細胞を用いて、骨芽細胞への分化や機能活性におけるMsx2の役割について検討を行った。Msx2を導入したES細胞及びiPS細胞とフィーダー細胞を共存培養し、その分化能や機能について検討するために分化関連遺伝子のmRNA発現やALP活性、さらに石灰化能等について調べた。結果より、ES細胞やiPS細胞から分化した骨芽細胞に対して、重要な役割を担うものと考えられた。","subitem_description_type":"Other"},{"subitem_description":"There have been no clear reports so far that Msx2 gene take part in the mechanism of osteoblast differentiation, which is responsible for the bone remodeling and regeneration in orthodontic treatments. The purpose of this study, therefore, was to investigate the roles of Msx2 in osteoblast differentiation and osteogenesis with mouse Embryonic Stem (ES) cells and Induced Pluripotent Stem (iPS) cells. ES cells and iPS cells which transfected with Msx2 gene were cocultured with feeder cells, and mRNA  leyels of osteoblastic phenotype and alkaline phosphatase activity were determined for cell differentiation and activation. The value of bone nodules was also determined for mineralization. As a result, it was suggested that Msx2 play a key role in controlling osteoblast differentiation from ES/iPS cells.","subitem_description_type":"Other"}]},"item_8_full_name_2":{"attribute_name":"著者よみ","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"ヤマモト, ヨシタケ"}]}]},"item_8_full_name_3":{"attribute_name":"別言語の著者","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"YAMAMOTO, Yoshitake"}]}]},"item_8_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学"}]},"item_8_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"491","subitem_subject_scheme":"NDC"},{"subitem_subject":"497","subitem_subject_scheme":"NDC"}]},"item_8_text_24":{"attribute_name":"公開者よみ","attribute_value_mlt":[{"subitem_text_value":"カゴシマ ダイガク"}]},"item_8_text_25":{"attribute_name":"公開者別名","attribute_value_mlt":[{"subitem_text_value":"Kagoshima University"}]},"item_8_text_41":{"attribute_name":"科研費番号 ","attribute_value_mlt":[{"subitem_text_value":"23792438"}]},"item_8_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山本, 芳丈"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-31"}],"displaytype":"detail","filename":"23792438_山本芳丈.pdf","filesize":[{"value":"86.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"23792438_山本芳丈.pdf","url":"https://ir.kagoshima-u.ac.jp/record/13245/files/23792438_山本芳丈.pdf"},"version_id":"056976a7-e04c-4e31-a741-8d30df31e556"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"骨芽細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞分化","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ES/iPS細胞へのMsx2遺伝子導入後の骨芽細胞への分化・機能活性に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ES/iPS細胞へのMsx2遺伝子導入後の骨芽細胞への分化・機能活性に関する研究"}]},"item_type_id":"8","owner":"3","path":["80"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-05-20"},"publish_date":"2015-05-20","publish_status":"0","recid":"13245","relation_version_is_last":true,"title":["ES/iPS細胞へのMsx2遺伝子導入後の骨芽細胞への分化・機能活性に関する研究"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-12-25T07:18:54.091862+00:00"}