{"created":"2023-07-25T08:12:49.720738+00:00","id":13267,"links":{},"metadata":{"_buckets":{"deposit":"0eec20fe-ddae-43be-9bcb-7f90d5b42771"},"_deposit":{"created_by":18,"id":"13267","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"13267"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00013267","sets":["57:86"]},"author_link":["124072"],"item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2015-09-14","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2014-07-11"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Medical Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"We recently showed using prepro-orexin knockout (ORX-KO) mice and orexin neuron-ablated (ORX-AB) mice that orexin neurons in the hypothalamus, but not orexin peptides per se, are indispensable for stress-induced thermogenesis. In order to examine whether orexin neurons are more generally involved in central thermoregulatory mechanisms or not, we applied other forms of thermogenic perturbations, including brain PGE2 injections which mimic inflammatory fever and environmental cold exposure, to ORX-KO mice, ORX-AB mice, and their wild-type (WT) litter mates. We found that ORX-AB mice, but not ORX-KO mice, exhibited a blunted PGE2-induced fever and intolerance to cold (5°C) exposure, and these findings were similar to the results previously obtained with stress-induced thermogenesis. PGE2-induced shivering was also attenuated in ORX-AB mice. Both mutants responded similarly to environmental heating (39°C). In WT and ORX-KO mice, the administration of PGE2 and cold exposure activated orexin neurons, as revealed by increased levels of expression of c-fos. Injection of retrograde tracer into the medullary raphe nucleus revealed direct and indirect projection from the orexin neurons, of which the latter seemed to be preserved in the ORX-AB mice. In addition, we found that glutamate receptor antagonists (D-(-)-2-amino-5-phosphonopentanoic acid and 6-cyano-7-nitroquinoxaline-2,3-dione) but not orexin receptor antagonists (SB334867 and OX2 29) successfully inhibited PGE2-induced fever in WT mice. These results suggest that orexin neurons are important in general thermogenic processes, and their importance is not restricted to stress-induced thermogenesis. In addition, these results indicate the possible involvement of glutamate in orexin neurons implicated in PGE2-induced fever.\n\nYoshiko Takahashi, Wei Zhang, Kohei Sameshima, Chiharu Kuroki, Ami Matsumoto, Jinko Sunanaga, Yu Kono, Takeshi Sakurai, Yuichi Kanmura, and Tomoyuki Kuwaki\nOrexin neurons are indispensable for prostaglandin E2-induced fever and defence against environmental cooling in mice\nJournal of Physiology, 2013, 591(Pt 22), p.5623–5643\ndoi: 10.1113/jphysiol.2013.261271","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲総研第297号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"Physiological Society","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"カゴシマ ダイガク","subitem_publisher_language":"ja-Kana"}]},"item_5_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_value":"【指導教員:上村裕一】"}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2014-04-02"}]},"item_5_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"高橋, 佳子","creatorNameLang":"ja"},{"creatorName":"タカハシ, ヨシコ","creatorNameLang":"ja-Kana"},{"creatorName":"Takahashi, Yoshiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-31"}],"displaytype":"detail","filename":"Diss_高橋_佳子_ISK297_2014.pdf","filesize":[{"value":"28.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Diss_高橋_佳子_ISK297_2014.pdf","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/13267/files/Diss_高橋_佳子_ISK297_2014.pdf"},"version_id":"15b93349-b179-4508-a960-e022287eec34"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-31"}],"displaytype":"detail","filename":"Abstract_高橋_佳子_4505810238_2014.pdf","filesize":[{"value":"90.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Abstract_高橋_佳子_4505810238_2014.pdf","objectType":"abstract","url":"https://ir.kagoshima-u.ac.jp/record/13267/files/Abstract_高橋_佳子_4505810238_2014.pdf"},"version_id":"0b9005af-69c6-4b65-91a7-4202689c0fb9"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Orexin neurons are indispensable for prostaglandin E2-induced fever and defence against environmental cooling in mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Orexin neurons are indispensable for prostaglandin E2-induced fever and defence against environmental cooling in mice","subitem_title_language":"en"}]},"item_type_id":"5","owner":"18","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-10-29"},"publish_date":"2015-10-29","publish_status":"0","recid":"13267","relation_version_is_last":true,"title":["Orexin neurons are indispensable for prostaglandin E2-induced fever and defence against environmental cooling in mice"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-09T01:41:11.272155+00:00"}