{"created":"2023-07-25T08:13:31.912888+00:00","id":14413,"links":{},"metadata":{"_buckets":{"deposit":"842aa467-c328-465e-9e95-191de20a82f4"},"_deposit":{"created_by":18,"id":"14413","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"14413"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00014413","sets":["57:86"]},"author_link":["132047"],"item_5_biblio_info_5":{"attribute_name":"収録雑誌名","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"368","bibliographicPageStart":"360","bibliographicVolumeNumber":"19","bibliographic_titles":[{"bibliographic_title":"INTERNATIONAL JOURNAL OF UROLOGY","bibliographic_titleLang":"en"}]}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2014-11-17"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Medical Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Objectives: Coincident with their worldwide use, resistance to fluoroquinolones in Escherichia coli has increased.To identify the gene expression profiles underlying fluoroquinolone resistance, we carried out genome-wide transcriptome analysis of fluoroquinolone-sensitiveE. coli.\nMethods: Four fluoroquinolone-sensitive E. coli and five fluoroquinolone-resistant E. coli clinical isolates were subjected to complementary deoxyribonucleic acid icroarray analysis. Some upregulated genes’ expression was verified by real-time polymerase chain reaction using 104 E. coli clinical isolates, and minimum inhibitory concentration tests were carried out by using their transformants.\nResults: A total of 40 genes were significantly upregulated influoroquinolone-resistant E. coli isolates (P<0.05). The expression of phage shock protein operons, which are involved in biofilm formation, was markedly upregulated in our profile of fluoroquinolone-resistant E. coli. One of the phage shock protein operons, pspC, was significantly upregulated in 50 fluoroquinolone-resistant E. coli isolates (P<0.0001). \nThe expression of type I fimbriae genes, which are pilus operons involved in biofilm formation, were markedly downregulated in fluoroquinolone-resistant E. coli. Deoxyri-bonucleic acid adenine methyltransferase (dam), which represses type I fimbriae genes, was significantly upregulated in the clinical fluoroquinolone-resistant E. coli iso-lates (P=0.007). We established pspC- and dam-expressing E. coli transformants from fluoroquinolone-sensitive E. coli, and the minimum inhibitory concentration tests showed that the transformants acquired fluoroquinolone resistance, suggesting that upregulation of these genes contributes to acquiring fluoroquinolone resistance.\nConclusions: Upregulation of psp operones and dam underlying pilus operons down-regulation might be associated with fluoroquinolone resistance in E. coli.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲総研第308号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"The Japanese Urological Association","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"カゴシマ ダイガク","subitem_publisher_language":"ja-Kana"}]},"item_5_relation_13":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"INTERNATIONAL JOURNAL OF UROLOGY"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://onlinelibrary.wiley.com/journal/14422042","subitem_relation_type_select":"URI"}}]},"item_5_relation_36":{"attribute_name":"関連（hasPart）","attribute_value_mlt":[{"subitem_relation_type":"hasPart","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1111/j.1442-2042.2011.02933.x","subitem_relation_type_select":"URI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2011 The Japanese Urological Association","subitem_rights_language":"en"}]},"item_5_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"491","subitem_subject_scheme":"NDC"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_value":"【指導教員:中川昌之】"}]},"item_5_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山根, 隆史","creatorNameLang":"ja"},{"creatorName":"ヤマネ, タカシ","creatorNameLang":"ja-Kana"},{"creatorName":"Yamane, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-08-01"}],"displaytype":"detail","filename":"Diss_山根_隆史_ISK308_2014.pdf","filesize":[{"value":"276.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Diss_山根_隆史_ISK308_2014","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/14413/files/Diss_山根_隆史_ISK308_2014.pdf"},"version_id":"73ee19da-f9ae-44f3-8a70-49c241054f0b"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-08-01"}],"displaytype":"detail","filename":"Abstract_山根_隆史_4504810387_2014.pdf","filesize":[{"value":"112.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Abstract_山根_隆史_4504810387_2014","objectType":"abstract","url":"https://ir.kagoshima-u.ac.jp/record/14413/files/Abstract_山根_隆史_4504810387_2014.pdf"},"version_id":"4e8a9dfa-91bd-419a-8137-e32dec521f25"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-08-02"}],"displaytype":"detail","filename":"Result_Yamane_Takashi_isk_308_2018.pdf","filesize":[{"value":"812.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Result_Yamane_Takashi_isk_308_2018","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/14413/files/Result_Yamane_Takashi_isk_308_2018.pdf"},"version_id":"7aa7d7d4-efef-497f-922c-ebd80b179f04"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-08-02"}],"displaytype":"detail","filename":"Comments_Yamane_Takashi_isk_308_2018.pdf","filesize":[{"value":"397.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Comments_Yamane_Takashi_isk_308_2018","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/14413/files/Comments_Yamane_Takashi_isk_308_2018.pdf"},"version_id":"af1598da-1bcb-430a-91e2-e03c516e0bbc"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Escherichia coli","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"fluoroquinolone resistance","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"microarray","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Genome-wide transcriptome analysis of fluoroquinolone resistance in clinical isolates of Escherichia coli","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Genome-wide transcriptome analysis of fluoroquinolone resistance in clinical isolates of Escherichia coli","subitem_title_language":"en"},{"subitem_title":"尿路由来大腸菌のフルオロキノロン耐性遺伝子についての解析","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"18","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-08-01"},"publish_date":"2018-08-01","publish_status":"0","recid":"14413","relation_version_is_last":true,"title":["Genome-wide transcriptome analysis of fluoroquinolone resistance in clinical isolates of Escherichia coli"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-09T01:46:38.364252+00:00"}