{"created":"2023-07-25T08:13:41.102898+00:00","id":14573,"links":{},"metadata":{"_buckets":{"deposit":"81957990-2bfb-47bd-8eaf-434cb80a369c"},"_deposit":{"created_by":18,"id":"14573","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"14573"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00014573","sets":["168:169"]},"author_link":["132842"],"item_5_biblio_info_5":{"attribute_name":"収録雑誌名","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"738","bibliographicPageStart":"727","bibliographicVolumeNumber":"224","bibliographic_titles":[{"bibliographic_title":"Brain Structure and Function","bibliographic_titleLang":"en"}]}]},"item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2019-03-05","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2019-03-25"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（保健学）","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Health Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"14-3-3γ is an important early ischemia-inducible protective factor against ischemic cell death in cerebral cortical neurons. We investigated the anti-apoptosis mechanism of enhanced 14-3-3γ mediated by preconditioning exercise-induced brain ischemic tolerance after stroke. Rats were assigned to 4 groups: exercise and ischemia (Ex group), ischemia and no exercise (No-Ex group), exercise and no ischemia (Ex-only group), and no exercise and ischemia (control group). Rats were trained on a treadmill for 5 days a week for 3 weeks (running speed, 25 m/min; running duration, 30 min/day). After the exercise program, stroke was induced by left middle cerebral artery occlusion. The infarct volume, neurological deficits, and motor function, as well as expression levels of hypoxiainduced factor-1α (HIF-1α), 14-3-3γ, P2X7 receptors, p-β-catenin Ser37, Bax, and caspase 3 were evaluated by immunohistochemistry and western blotting. The expression of HIF-1α and 14-3-3γ significantly increased in neurons and astrocytes in the Ex-only group. HIF-1α was co-expressed with P2X7 receptor- and GFAP-positive astrocytes. After stroke, the Ex group had significantly reduced brain infarction. HIF-1α and 14-3-3γ significantly increased in the Ex group compared to the No-Ex group. In addition, p-β-catenin Ser37 significantly increased following elevated 14-3-3γ; in contrast, Bax and caspase 3 were significantly reduced in the Ex group. Our findings suggest that preconditioning exercise prior to ischemia induces neuron- and astrocyte-mediated brain ischemic tolerance through increased expression of HIF-1α and 14-3-3γ, which are intrinsic protective factors; the upregulated 14-3-3γ induced by preconditioning exercise reduces ischemic neuronal cell death through the 14-3-3γ/p-β-catenin Ser37/Bax/caspase 3 anti-apoptotic pathway.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲保研第13号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"Springer","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"カゴシマ ダイガク","subitem_publisher_language":"ja-Kana"}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s00429-018-1800-4","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© Springer-Verlag GmbH Germany, part of Springer Nature 2018"}]},"item_5_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"498","subitem_subject_scheme":"NDC"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_value":"【指導教員：榊間春利】 "}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2018-12-13"}]},"item_5_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大塚, 章太郎","creatorNameLang":"ja"},{"creatorName":"オオツカ, ショウタロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Otsuka, Shotaro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"132842","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-12-03"}],"displaytype":"detail","filename":"Diss_大塚_章太郎_HOK013_2018.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Diss_大塚_章太郎_HOK013_2018","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/14573/files/Diss_大塚_章太郎_HOK013_2018.pdf"},"version_id":"bb4aa317-441d-4b99-999e-8ae93d5eceff"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-22"}],"displaytype":"detail","filename":"Abstract_大塚_章太郎_4716700015_2018.pdf","filesize":[{"value":"203.3 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kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Comments_Otsuka_Shotaro_hok13_2019","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/14573/files/Comments_Otsuka_Shotaro_hok13_2019.pdf"},"version_id":"df682989-8436-4cb9-a241-9d9655322093"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"14-3-3γ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"HIF-1α","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"physical activity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ischemic tolerance","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"intrinsic factor","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Preconditioning exercise reduces brain damage and neuronal apoptosis through enhanced endogenous 14-3-3γ after focal brain ischemia in rats","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Preconditioning exercise reduces brain damage and neuronal apoptosis through enhanced endogenous 14-3-3γ after focal brain ischemia in rats","subitem_title_language":"en"},{"subitem_title":"脳梗塞発症前運動は、ラットの局所脳虚血後、内在性14-3-3γの増強を介して脳損傷および神経細胞のアポトーシスを減少させる","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"18","path":["169"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-03-25"},"publish_date":"2019-03-25","publish_status":"0","recid":"14573","relation_version_is_last":true,"title":["Preconditioning exercise reduces brain damage and neuronal apoptosis through enhanced endogenous 14-3-3γ after focal brain ischemia in rats"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-08T23:55:11.321257+00:00"}