{"created":"2023-07-25T08:13:44.184065+00:00","id":14628,"links":{},"metadata":{"_buckets":{"deposit":"1611e63e-2e6c-49fb-beb2-1073ceb948bc"},"_deposit":{"created_by":18,"id":"14628","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"14628"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00014628","sets":["57:86"]},"author_link":["133086"],"item_5_biblio_info_5":{"attribute_name":"収録雑誌名","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"613","bibliographicPageStart":"608","bibliographicVolumeNumber":"508","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications","bibliographic_titleLang":"en"}]}]},"item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2019-03-07","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2019-03-19"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Medical Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Background: Inhalation of aerosolized Legionella pneumophila, a Gram-negative bacterium, can cause severe pneumonia. During infection, L. pneumophila replicates intracellularly in macrophages. The involvement of host micro RNAs (miRNAs) in L. pneumophila infection is not fully understood.\nMethods: The human macrophage-like cell line U937 was infected with L. pneumophila. The levels of miRNA and messenger RNA (mRNAs) were measured using reverse transcriptase polymerase chain reaction. Release of lactate dehydrogenase was used to evaluate cytotoxicity The expression of RICTOR and related proteins was examined by western blotting of cell lysates.\nResults: L. pneumophila infection upregulated the expression of miR-218 and the host genes SLIT2 and SLIT3 in U937 cells. The expression of RICTOR, a component of the mechanistic target of rapamycin complex 2 (mTORC2), decreased during L. pneumophila infection. RICTOR protein expression was inhibited by the overexpression of miR-218, whereas knockdown of miR-218 restored the downregulation of RICTOR by L. pneumophila . L. pneumophila infection induced the expression of the proinflammatory cytokines IL-6 and TNF alpha, which was modulated by knockdown of miR-218 or RICTOR.\nConclusions: Our study revealed the involvement of miR-218 in regulating the inflammatory response of macrophages against L. pneumophila infection. These findings suggest potential novel roles for miR-218 and RICTOR as therapeutic targets of L. pneumophila infection.\n","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲総研第493号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier Inc.","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"カゴシマ ダイガク","subitem_publisher_language":"ja-Kana"}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.bbrc.2018.11.093","subitem_relation_type_select":"DOI"}}]},"item_5_relation_13":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Biochemical and Biophysical Research Communications"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/journal/biochemical-and-biophysical-research-communications","subitem_relation_type_select":"URI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2018 Elsevier Inc. All rights reserved.","subitem_rights_language":"en"}]},"item_5_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"491","subitem_subject_scheme":"NDC"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_value":"【指導教員:橋口照人】"}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2019-02-06"}]},"item_5_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"郡山, 豊泰","creatorNameLang":"ja"},{"creatorName":"コオリヤマ, トヨヤス","creatorNameLang":"ja-Kana"},{"creatorName":"Koriyama, Toyoyasu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"133086","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-12-11"}],"displaytype":"detail","filename":"Diss_郡山_豊泰_ISK493_2018（著者最終稿）.pdf","filesize":[{"value":"901.5 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"Diss_郡山_豊泰_ISK493_2018","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/14628/files/Diss_郡山_豊泰_ISK493_2018（著者最終稿）.pdf"},"version_id":"51c4d757-de94-410d-8cb1-16c232670965"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-04-16"}],"displaytype":"detail","filename":"Abstract_郡山_豊泰_4511810270_2018.pdf","filesize":[{"value":"30.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Abstract_郡山_豊泰_4511810270_2018","objectType":"abstract","url":"https://ir.kagoshima-u.ac.jp/record/14628/files/Abstract_郡山_豊泰_4511810270_2018.pdf"},"version_id":"d1ad87fd-07ce-41a8-bbac-587875d621e0"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-04-22"}],"displaytype":"detail","filename":"Result_Koriyama_Toyoyasu_isk_493.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Result_Koriyama_Toyoyasu_isk_493","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/14628/files/Result_Koriyama_Toyoyasu_isk_493.pdf"},"version_id":"e7c277e8-2427-44df-a4b9-4c669074a355"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-04-22"}],"displaytype":"detail","filename":"Comments_Koriyama_Toyoyasu_isk_493.pdf","filesize":[{"value":"580.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Comments_Koriyama_Toyoyasu_isk_493","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/14628/files/Comments_Koriyama_Toyoyasu_isk_493.pdf"},"version_id":"76a796ba-d90e-4eb5-a277-b7bee3fcdfcc"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"microRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Legionella","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Rictor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"macrophage","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Legionella pneumophila infection-mediated regulation of RICTOR via miR-218 in U937 macrophage cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Legionella pneumophila infection-mediated regulation of RICTOR via miR-218 in U937 macrophage cells","subitem_title_language":"en"},{"subitem_title":"Legionella pneumophilaは、マクロファージ様U937細胞に感染するとmiR-218を介してRICTORを制御する","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"18","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-04-16"},"publish_date":"2019-04-16","publish_status":"0","recid":"14628","relation_version_is_last":true,"title":["Legionella pneumophila infection-mediated regulation of RICTOR via miR-218 in U937 macrophage cells"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-09T01:49:16.802108+00:00"}