{"created":"2023-07-25T08:13:44.269777+00:00","id":14630,"links":{},"metadata":{"_buckets":{"deposit":"92ef34a5-6654-4def-a90a-66833084a602"},"_deposit":{"created_by":18,"id":"14630","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"14630"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00014630","sets":["57:86"]},"author_link":["133092"],"item_5_biblio_info_5":{"attribute_name":"収録雑誌名","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"274","bibliographicPageStart":"266","bibliographicVolumeNumber":"27","bibliographic_titles":[{"bibliographic_title":"Journal of Food and Drug Analysis","bibliographic_titleLang":"en"}]}]},"item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2019-03-07","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2019-03-19"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Medical Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Rubiscolin-6 is an opioid peptide derived from plant ribulose bisphosphate carboxylase/oxygenase (Rubisco). It has been demonstrated that opioid receptors could control glucose homeostasis in skeletal muscle independent of insulin action. Therefore, Rubiscolin-6 may be involved in the control of glucose metabolism. In the present study, we investigated the effect of rubiscolin-6 on glucose uptake in skeletal muscle. Rubiscolin-6-induced glucose uptake was measured using the fluorescent indicator 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxyglucose (2-NBDG) in L6 and C2C12 cell lines. The protein expressions of glucose transporter 4 (GLUT4) and AMP-activated protein kinase (AMPK) in L6 cells were observed by Western blotting. The in vivo effects of rubiscolin-6 were characterized in streptozotocin (STZ)-induced diabetic rats. Rubiscolin-6 induced a concentration-dependent increase in glucose uptake levels. The increase of phospho-AMPK (pAMPK) and GLUT4 expressions were also observed in L6 and C2C12 cells. Effects of rubiscolin-6 were blocked by opioid receptor antagonists and/or associated signals inhibitors. Moreover, Rubiscolin-6 produced a dose-dependent reduction of blood glucose and increased GLUT4 expression in STZ-induced diabetic rats. In conclusion, rubiscolin-6 increases glucose uptake, potentially via an activation of AMPK to enhance GLUT4 translocation after binding to opioid receptors in skeletal muscle.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲総研第495号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"Food and Drug Administration","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"カゴシマ ダイガク","subitem_publisher_language":"ja-Kana"}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.jfda.2018.06.012","subitem_relation_type_select":"DOI"}}]},"item_5_relation_13":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Journal of Food and Drug Analysis"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.jfda-online.com/","subitem_relation_type_select":"URI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2018 Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC.","subitem_rights_language":"en"}]},"item_5_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"491","subitem_subject_scheme":"NDC"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_value":"【指導教員:浅川 明弘】"}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2018-10-03"}]},"item_5_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kairupan, Timothy Sean","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"133092","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-12-11"}],"displaytype":"detail","filename":"Diss_Kairupan_Timothy_ISK495_2018（著者最終稿）.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"Diss_Kairupan_Timothy_ISK495_2018","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/14630/files/Diss_Kairupan_Timothy_ISK495_2018（著者最終稿）.pdf"},"version_id":"44873f85-fbfb-4534-b778-985d9c2f7ed8"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-04-16"}],"displaytype":"detail","filename":"Abstract_Kairupan_Timothy_4514800080_2018.pdf","filesize":[{"value":"38.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Abstract_Kairupan_Timothy_4514800080_2018","objectType":"abstract","url":"https://ir.kagoshima-u.ac.jp/record/14630/files/Abstract_Kairupan_Timothy_4514800080_2018.pdf"},"version_id":"0a4c94aa-d6a6-4f5a-bf65-d7f309c3de7f"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-04-22"}],"displaytype":"detail","filename":"Result_Timothy_Sean_Kairupan_isk_495.pdf","filesize":[{"value":"882.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Result_Timothy_Sean_Kairupan_isk_495","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/14630/files/Result_Timothy_Sean_Kairupan_isk_495.pdf"},"version_id":"a3d98ba3-9cd9-4708-ac8c-021d53e7ef61"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-04-22"}],"displaytype":"detail","filename":"Comments_Timothy_Sean_Kairupan_isk_495.pdf","filesize":[{"value":"419.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Comments_Timothy_Sean_Kairupan_isk_495","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/14630/files/Comments_Timothy_Sean_Kairupan_isk_495.pdf"},"version_id":"cba2b44e-4050-4bf6-b60b-13febe2647d9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"AMPK","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Glucose Uptake","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"GLUT4","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Opioid Receptor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Rubiscolin-6","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Rubiscolin-6 activates opioid receptors to enhance glucose uptake in skeletal muscle","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Rubiscolin-6 activates opioid receptors to enhance glucose uptake in skeletal muscle","subitem_title_language":"en"},{"subitem_title":"ルビスコリン-６はオピオイド受容体を活性化することで骨格筋の糖取込みを促進する","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"18","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-04-16"},"publish_date":"2019-04-16","publish_status":"0","recid":"14630","relation_version_is_last":true,"title":["Rubiscolin-6 activates opioid receptors to enhance glucose uptake in skeletal muscle"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-09T01:49:22.360805+00:00"}