{"created":"2023-07-25T08:13:49.077703+00:00","id":14730,"links":{},"metadata":{"_buckets":{"deposit":"b8cfc968-d51b-4383-9206-8b5b2adbe161"},"_deposit":{"created_by":18,"id":"14730","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"14730"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00014730","sets":["57:86"]},"author_link":["133562"],"item_5_biblio_info_5":{"attribute_name":"収録雑誌名","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{"bibliographic_title":"Auris Nasus Larynx","bibliographic_titleLang":"en"}]}]},"item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2019-04-10","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2019-04-25"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Medical Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Objective: Phosphorylcholine (PC) is a structural component of Streptococcus pneumoniae (Spn) and nontypeable Haemophilus influenzae (NTHi), and is known to be associated with adherence through the platelet activating factor receptor (PAF-R). Furthermore, high PC expression is considered to be involved in Spn and NTHi virulence. In this study, we examined the influence of PC expression on the adherence of Spn and NTHi to epithelial cells in order to clarify the potential effectiveness of a vaccine targeting PC.\nMethods: Twenty-seven strains of Spn and twenty-two strains of NTHi were used, cultured overnight, and PC expression was evaluated by fluorescence activated cell sorting; the strains were divided into two groups: PC low expression (PC-low) and PC high expression (PC-high) groups. Bacterial adherence was then examined using Detroit 562 cells and BALB/c mice. Bacterial invasion was then examined in Detroit 562 cells.\nResults: The adherence of Spn and NTHi and invasion of NTHi in the PC-high group was significantly reduced by pretreatment with a monoclonal anti-PC antibody (TEPC-15), PAF-R antagonist (ABT-491), and PC-keyhole limpet hemocyanin (PC-KLH). However, such findings were not observed in the PC-low group.\nConclusion: The present study suggests that PC is involved in the mucosal adhesion of Spn and NTHi, and the mucosal invasion of NTHi with PC-high strains, but not PC-low strains. These results suggest that a PC-targeting mucosal vaccine only affects PC-high Spn and NTHi strains and does not disturb commensal bacterial flora in the upper respiratory tract, which comprises nonpathogenic PClow bacteria.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲総研第509号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"elsevier","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学 ","subitem_publisher_language":"ja"},{"subitem_publisher":"カゴシマ ダイガク","subitem_publisher_language":"ja-Kana"}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.anl.2018.11.003","subitem_relation_type_select":"DOI"}}]},"item_5_relation_13":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Auris Nasus Larynx"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.journals.elsevier.com/auris-nasus-larynx/","subitem_relation_type_select":"URI"}}]},"item_5_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"491","subitem_subject_scheme":"NDC"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_value":"【指導教員:黒野祐一】 "}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2019-02-06 "}]},"item_5_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"井内, 寛之","creatorNameLang":"ja"},{"creatorName":"イウチ, ヒロユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Iuchi, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-22"}],"displaytype":"detail","filename":"Diss_井内_寛之_ISK509_2019.pdf","filesize":[{"value":"849.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Diss_井内_寛之_ISK509_2019","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/14730/files/Diss_井内_寛之_ISK509_2019.pdf"},"version_id":"50b4c1b0-9fd1-4496-862c-b0a79911da35"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-22"}],"displaytype":"detail","filename":"Abstract_井内_寛之_4514810040_2018.pdf","filesize":[{"value":"158.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Abstract_井内_寛之_4514810040_2018","objectType":"abstract","url":"https://ir.kagoshima-u.ac.jp/record/14730/files/Abstract_井内_寛之_4514810040_2018.pdf"},"version_id":"9c564ccc-f72c-4937-88fe-48a3f532b7f8"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-05-23"}],"displaytype":"detail","filename":"Result_Iuchi_Hiroyuki_isk_509.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Result_Iuchi_Hiroyuki_isk_509","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/14730/files/Result_Iuchi_Hiroyuki_isk_509.pdf"},"version_id":"30886987-731d-4368-9560-4ce639e75a2a"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-05-23"}],"displaytype":"detail","filename":"Comments_Iuchi_Hiroyuki_isk_509.pdf","filesize":[{"value":"533.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Comments_Iuchi_Hiroyuki_isk_509","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/14730/files/Comments_Iuchi_Hiroyuki_isk_509.pdf"},"version_id":"bfbd55ff-8d62-45e5-8abc-d827c1f9f2ca"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Phosphorylcholine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Streptococcus pneumoniae","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Nontypeable Haemophilus influenza","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PAF-R","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Role of phospharylcholine in Streptococcus pneumoniae and nontypeable Haemophilus influenzae adherence to epithelial cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Role of phospharylcholine in Streptococcus pneumoniae and nontypeable Haemophilus influenzae adherence to epithelial cells","subitem_title_language":"en"},{"subitem_title":"肺炎球菌と無萊膜インフルエンザ菌の上皮細胞接着におけるホスホリルコリンの役割","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"18","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-05-23"},"publish_date":"2019-05-23","publish_status":"0","recid":"14730","relation_version_is_last":true,"title":["Role of phospharylcholine in Streptococcus pneumoniae and nontypeable Haemophilus influenzae adherence to epithelial cells"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-09T01:50:05.768588+00:00"}