{"created":"2023-07-25T08:14:01.584693+00:00","id":14959,"links":{},"metadata":{"_buckets":{"deposit":"40c3ac53-0002-4ca3-8297-3bd1e7d5f99f"},"_deposit":{"created_by":18,"id":"14959","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"14959"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00014959","sets":["57:86"]},"author_link":["134658"],"item_5_biblio_info_5":{"attribute_name":"収録雑誌名","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-03-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"Cancers","bibliographic_titleLang":"en"}]}]},"item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2019-12-06","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2019-12-18"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Medical Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Previously, we established a microRNA (miRNA) expression signature in pancreatic ductal adenocarcinoma (PDAC) tissues using RNA sequencing and found significantly reduced expression of miR-204-5p. Here, we aimed to investigate the functional significance of miR-204-5p and to identify miR-204-5p target genes involved in PDAC pathogenesis. Cancer cell migration and invasion were significantly inhibited by ectopic expression of miR-204-5p in PDAC cells. Comprehensive gene expression analyses and in silico database searches revealed 25 putative targets regulated by miR-204-5p in PDAC cells. Among these target genes, high expression levels of RACGAP1, DHRS9, AP1S3, FOXC1, PRP11, RHBDL2 and MUC4 were significant predictors of a poor prognosis of patients with PDAC. In this study, we focused on RACGAP1 (Rac guanosine triphosphatase-activating protein 1) because its expression was most significantly predictive of PDAC pathogenesis (overall survival rate: p = 0.0000548; disease-free survival rate: p = 0.0014). Overexpression of RACGAP1 was detected in PDAC clinical specimens, and its expression enhanced the migration and invasion of PDAC cells. Moreover, downstream genes affected by RACGAP1 (e.g., MMP28, CEP55, CDK1, ANLN and S100A14) are involved in PDAC pathogenesis. Our strategy to identify antitumor miRNAs and their target genes will help elucidate the molecular pathogenesis of PDAC.\n\n\nMuhammad Khalid, Tetsuya Idichi, Naohiko Seki, Masumi Wada, Yasutaka Yamada, Haruhi Fukuhisa, Hiroko Toda, Yoshiaki Kita, Yota Kawasaki, Kiyonori Tanoue, Hiroshi Kurahara, Yuko Mataki, Kosei Maemura and Shoji Natsugoe\nGene Regulation by Antitumor miR-204-5p in Pancreatic Ductal Adenocarcinoma: The Clinical Significance of Direct RACGAP1 Regulation\nCancers 2019, 11(3), 327\ndoi: https://doi.org/10.3390/cancers11030327 ","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲総研第529号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"カゴシマ ダイガク","subitem_publisher_language":"ja-Kana"}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/cancers11030327","subitem_relation_type_select":"DOI"}}]},"item_5_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_value":"【指導教員:夏越祥次】 "}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2019-06-05"}]},"item_5_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Khalid Muhammad","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-10-23"}],"displaytype":"detail","filename":"Diss_Khalid_Muhammad_ISK000_2019.pdf","filesize":[{"value":"3.9 MB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"Diss_Khalid_Muhammad_ISK000_2019","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/14959/files/Diss_Khalid_Muhammad_ISK000_2019.pdf"},"version_id":"ad47c735-2ae7-4997-927d-c4edf57a7582"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-10-23"}],"displaytype":"detail","filename":"Abstract_ 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kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Result_Khalid_Muhammad_isk_529_2019","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/14959/files/Result_Khalid_Muhammad_isk_529_2019.pdf"},"version_id":"c7061861-1dad-41f7-94d2-b66a5e2b8339"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-01-27"}],"displaytype":"detail","filename":"Comments_Khalid_Muhammad_isk_529.pdf","filesize":[{"value":"492.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Comments_Khalid_Muhammad_isk_529","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/14959/files/Comments_Khalid_Muhammad_isk_529.pdf"},"version_id":"771ad0b6-df64-4a96-a555-1c194c5409a5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"microRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"miR-204-5p","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"antitumor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"pancreatic ductal adenocarcinoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"pathogenesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RACGAP1","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Gene Regulation by Antitumor miR-204-5p in Pancreatic Ductal Adenocarcinoma : The Clinical Significance of Direct RACGAP1 Regulation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Gene Regulation by Antitumor miR-204-5p in Pancreatic Ductal Adenocarcinoma : The Clinical Significance of Direct RACGAP1 Regulation","subitem_title_language":"en"},{"subitem_title":"癌抑制型マイクロRNA(miR-204-5p)が制御する膵臓癌 : RACGAP1の臨床的意義","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"18","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-01-27"},"publish_date":"2020-01-27","publish_status":"0","recid":"14959","relation_version_is_last":true,"title":["Gene Regulation by Antitumor miR-204-5p in Pancreatic Ductal Adenocarcinoma : The Clinical Significance of Direct RACGAP1 Regulation"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-09T01:51:15.372333+00:00"}