{"created":"2023-07-25T08:14:03.131253+00:00","id":14993,"links":{},"metadata":{"_buckets":{"deposit":"92c571ff-1bb4-4f74-a86d-785d88011c1a"},"_deposit":{"created_by":18,"id":"14993","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"14993"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00014993","sets":["57:86"]},"author_link":["134819"],"item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2020-01-14","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2020-01-28"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(歯学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Dental Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Streptococcus mutans is a cariogenic bacterium that localizes in the oral cavity. Glycyrrhetinic acid (GRA) is a major component of licorice extract. GRA and several derivatives, including disodium succinoyl glycyrrhetinate (GR-SU), are known to have anti-inflammatory effects in humans. In this study, the antimicrobial effect of GRA and its derivatives against the S. mutans UA159 strain were investigated. Minimum inhibitory concentrations (MICs) of GRA and GR-SU showed antibacterial activity against the S. mutans strain, whereas other tested derivatives did not. Because GR-SU is more soluble than GRA, GR-SU was used for further experiments. The antibacterial activity of GR-SU against 100 S. mutans strains was evaluated and it was found that all strains are susceptible to GR-SU, with MIC values below 256 µg/mL. A cell viability assay showed that GR-SU has a bacteriostatic effect on S. mutans cells. As to growth kinetics, sub-MICs of GR-SU inhibited growth. The effect of GR-SU on S. mutans virulence was then investigated. GR-SU at sub-MICs suppresses biofilm formation. Additionally, GR-SU greatly suppresses the pH drop caused by the addition of glucose and glucose-induced expression of the genes responsible for acid production (ldh and pykF) and tolerance (aguD and atpD). Additionally, expression of enolase, which is responsible for the carbohydrate phosphotransferase system, was not increased in the presence of GR-SU, indicating that GR-SU suppresses incorporation of sugars into S. mutans. In conclusion, GR-SU has antibacterial activity against S. mutans and also decreases S. mutans virulence.\n\nThis is the peer reviewed version of the following article:\nTakahito Yamashita, Miki Kawada-Matsuo, Tamaki Katsumata, Atsuko Watanabe, Yuichi Oogai, Yoshihiro Nishitani, Shouichi Miyawaki, Hitoshi Komatsuzawa.\nSafety, speed, and effectiveness of air transportation for neonates\nMicrobiology and immunology (2019), 63(7), 251-260\n© 2019 The Societies and John Wiley & Sons Australia, Ltd\nwhich has been published in final form at  https://doi.org/10.1111/1348-0421.12717\nThis article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲総研第532号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"The Societies and John Wiley & Sons Australia, Ltd","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"カゴシマ ダイガク","subitem_publisher_language":"ja-Kana"}]},"item_5_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_value":"【指導教員:仙波伊知郎/松尾美樹】 "}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2019-09-04"}]},"item_5_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山下, 剛史","creatorNameLang":"ja"},{"creatorName":"ヤマシタ, タカヒト","creatorNameLang":"ja-Kana"},{"creatorName":"Yamashita, Takahito","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"134819","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-08-04"}],"displaytype":"detail","filename":"Diss_山下_剛史_ISK532_2019.pdf","filesize":[{"value":"902.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Diss_山下_剛史_ISK532_2019","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/14993/files/Diss_山下_剛史_ISK532_2019.pdf"},"version_id":"ae494ce5-9954-47bf-ab1b-fecfb4881df9"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-03"}],"displaytype":"detail","filename":"Abstract_山下_剛史_4512800142_2019.pdf","filesize":[{"value":"146.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Abstract_山下_剛史_4512800142_2019","objectType":"abstract","url":"https://ir.kagoshima-u.ac.jp/record/14993/files/Abstract_山下_剛史_4512800142_2019.pdf"},"version_id":"4bea3d8e-86a5-43ae-b5d4-0f262505c1b8"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-13"}],"displaytype":"detail","filename":"Result_Yamashita_Takahito_isk_532.pdf","filesize":[{"value":"556.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Result_Yamashita_Takahito_isk_532","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/14993/files/Result_Yamashita_Takahito_isk_532.pdf"},"version_id":"450f9bb2-34be-47d8-bd78-b803cc48210f"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-13"}],"displaytype":"detail","filename":"Comments_Yamashita_Takahito_isk_532.pdf","filesize":[{"value":"225.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Comments_Yamashita_Takahito_isk_532","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/14993/files/Comments_Yamashita_Takahito_isk_532.pdf"},"version_id":"4dc2e02d-e4c1-4ff6-8320-97836b15ac63"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Antibacterial agent","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Streptococcus mutans","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Antibacterial activity of disodium succinoyl glycyrrhetinate, a derivative of glycyrrhetinic acid against Streptococcus mutans","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Antibacterial activity of disodium succinoyl glycyrrhetinate, a derivative of glycyrrhetinic acid against Streptococcus mutans","subitem_title_language":"en"},{"subitem_title":"グリチルレチン酸誘導体であるサクシニルグリチルレチン酸ニナトリウムのStreptococcus mutansに対する抗菌効果について","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"18","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-03-13"},"publish_date":"2020-03-13","publish_status":"0","recid":"14993","relation_version_is_last":true,"title":["Antibacterial activity of disodium succinoyl glycyrrhetinate, a derivative of glycyrrhetinic acid against Streptococcus mutans"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-09T01:51:23.569390+00:00"}