{"created":"2023-07-25T08:14:03.446821+00:00","id":15000,"links":{},"metadata":{"_buckets":{"deposit":"b1c65a1e-659d-4102-b71a-843ee3136e7a"},"_deposit":{"created_by":18,"id":"15000","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"15000"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00015000","sets":["168:169"]},"author_link":["134838"],"item_5_biblio_info_5":{"attribute_name":"収録雑誌名","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{"bibliographic_title":"Journal of Neuroinflammation","bibliographic_titleLang":"en"}]}]},"item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2020-03","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2020-03-25"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（保健学）","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Health Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Background: Midkine (MK) is a multifunctional cytokine found upregulated in the brain in the presence of different disorders characterized by neuroinflammation, including neurodegenerative disorders and ischemia. The neuroinflammatory response to traumatic brain injury (TBI) represents a key secondary injury factor that can result in further neuronal injury. In the present study, we investigated the role of endogenous MK in secondary injury, including neuroinflammation, immune response, and neuronal apoptosis activity, after TBI.\nMethods: Wild type (Mdk+/+) and MK gene deficient (Mdk−/−) mice were subjected to fluid percussion injury for TBI models and compared at 3, 7, and 14 days after TBI, in terms of the following: brain tissue loss, neurological deficits, microglia response, astrocytosis, expression of proinflammatory M1 and anti-inflammatory M2 microglia/macrophage phenotype markers, and apoptotic activity.\nResults: As opposed to Mdk+/+ mice, Mdk−/− mice reported a significantly reduced area of brain tissue loss and an improvement in their neurological deficits. The ratios of the Iba1-immunoreactive microglia/macrophages in the perilesional site were significantly decreased in Mdk−/− than in the Mdk+/+ mice at 3 days after TBI. However, the ratios of the glial fibrillary acidic protein immunoreactive area were similar between the two groups. The M1 phenotype marker (CD16/32) immunoreactive areas were significantly reduced in Mdk−/− than in the Mdk+/+ mice. Likewise, the mRNA levels of the M1 phenotype markers (TNF-α, CD11b) were significantly decreased in Mdk−/− mice than in Mdk+/+ mice. Furthermore, flow cytometry analysis identified the M2 markers, i.e., CD163+ macrophages cells and arginase-1+ microglia cells, to be significantly higher in Mdk−/− than in Mdk+/+ mice. Finally, the ratios of apoptotic neurons were significantly decreased in the area surrounding the lesion in Mdk−/− than in Mdk+/+ mice following TBI.\nConclusion: Our findings suggest that MK-deficiency reduced tissue infiltration of microglia/macrophages and altered their polarization status thereby reducing neuroinflammation, neuronal apoptosis, and tissue loss and improving neurological outcomes after TBI. Therefore, targeting MK to modulate neuroinflammation may represent a potential therapeutic strategy for TBI management.\n\nSeiya Takada, Harutoshi Sakakima, Takahiro Matsuyama, Shotaro Otsuka, Kazuki Nakanishi, Kosuke Norimatsu, Yuki Itashiki, Akira Tani and Kiyoshi Kikuchi\nDisruption of Midkine gene reduces traumatic brain injury through the modulation of neuroinflammation\nJournal of Neuroinflammation (2020)","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲保研第18号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"BMC Part of Springer Nature","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"カゴシマ ダイガク","subitem_publisher_language":"ja-Kana"}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1186/s12974-020-1709-8","subitem_relation_type_select":"DOI"}}]},"item_5_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_value":"【指導教員：榊間春利】"}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2020-01-16"}]},"item_5_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"髙田, 聖也","creatorNameLang":"ja"},{"creatorName":"タカダ, セイヤ","creatorNameLang":"ja-Kana"},{"creatorName":"Takada, Seiya","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-17"}],"displaytype":"detail","filename":"Diss_髙田_聖也_HOK018_2019.pdf","filesize":[{"value":"2.0 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kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Result_Takada_Seiya_hok_18","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/15000/files/Result_Takada_Seiya_hok_18.pdf"},"version_id":"71463423-ca9e-436b-af05-c613ecce3c86"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-17"}],"displaytype":"detail","filename":"Comments_Takada_Seiya_hok_18.pdf","filesize":[{"value":"392.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Comments_Takada_Seiya_hok_18","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/15000/files/Comments_Takada_Seiya_hok_18.pdf"},"version_id":"1ac12936-16f7-4b59-992a-ba8a7b90ff07"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Midkine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Traumatic brain injury","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Microglia/macrophages","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"M1/M2 phenotype","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Neuroinflammation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Disruption of Midkine gene reduces traumatic brain injury through the modulation of neuroinflammation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Disruption of Midkine gene reduces traumatic brain injury through the modulation of 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