{"created":"2023-07-25T08:14:12.292844+00:00","id":15187,"links":{},"metadata":{"_buckets":{"deposit":"f275b111-aec9-4eaf-a87e-9546184a5ea1"},"_deposit":{"created_by":18,"id":"15187","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"15187"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00015187","sets":["57:86"]},"author_link":["135849"],"item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2020-03-06","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2020-03-12"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Medical Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"In the human genome, miR-451a is encoded close to the miR-144 on chromosome region 17q11.2. Our previous study showed that both strands of pre-miR-144 acted as antitumor miRNAs and were involved in lung squamous cell carcinoma (LUSQ) pathogenesis. Here, we aimed to investigate the functional significance of miR-451a and to identify its targeting of oncogenic genes in LUSQ cells. Downregulation of miR-451a was confirmed in LUSQ clinical specimens, and low expression of miR-451a was significantly associated with poor prognosis of LUSQ patients (overall survival: p = 0.035, disease-free survival: p = 0.029). Additionally, we showed that ectopic expression of miR-451a significantly blocked cancer cell aggressiveness. In total, 15 putative oncogenic genes were shown to be regulated by miR-451a in LUSQ cells. Among these targets, high kinesin family member 2A (KIF2A) expression was significantly associated with poor prognosis (overall survival: p = 0.043, disease-free survival: p = 0.028). Multivariate analysis showed that KIF2A expression was an independent prognostic factor in patients with LUSQ (hazard ratio = 1.493, p = 0.034). Aberrant KIF2A expression promoted the malignant transformation of this disease. Analytic strategies based on antitumor miRNAs and their target oncogenes are effective tools for identification of novel molecular pathogenesis of LUSQ.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲総研第557号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"カゴシマ ダイガク","subitem_publisher_language":"ja-Kana"}]},"item_5_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_value":"【指導教員:井上博雅】"}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2019-10-02"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"内田, 章文","creatorNameLang":"ja"},{"creatorName":"ウチダ, アキフミ","creatorNameLang":"ja-Kana"},{"creatorName":"Uchida, Akifumi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-05"}],"displaytype":"detail","filename":"Abstract_内田_章文_4517810052_2019.pdf","filesize":[{"value":"26.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Abstract_内田_章文_4517810052_2019","objectType":"abstract","url":"https://ir.kagoshima-u.ac.jp/record/15187/files/Abstract_内田_章文_4517810052_2019.pdf"},"version_id":"1740376e-67da-45a5-8913-4f970481872f"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-05-21"}],"displaytype":"detail","filename":"Result_Uchida_Akifumi_isk_557.pdf","filesize":[{"value":"255.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Result_Uchida_Akifumi_isk_557","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/15187/files/Result_Uchida_Akifumi_isk_557.pdf"},"version_id":"2a6a23cc-5cfa-4083-a844-c65cf5ce76dc"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-05-21"}],"displaytype":"detail","filename":"Comments_Uchida_Akifumi_isk_557.pdf","filesize":[{"value":"101.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Comments_Uchida_Akifumi_isk_557","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/15187/files/Comments_Uchida_Akifumi_isk_557.pdf"},"version_id":"c7a0568c-27f9-4003-b6f8-1be224e84016"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"microRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"miR-451a","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"KIF2A","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"lung squamous cell carcinoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"antitumor","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Regulation of KIF2A by Antitumor miR-451a Inhibits Cancer Cell Aggressiveness Features in Lung Squamous Cell  Carcinoma","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Regulation of KIF2A by Antitumor miR-451a Inhibits Cancer Cell Aggressiveness Features in Lung Squamous Cell  Carcinoma","subitem_title_language":"en"},{"subitem_title":"肺扁平上皮癌において癌抑制型 miR-451a はKIF2A を制御して肺癌細胞の進展を抑制する","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"18","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-05-21"},"publish_date":"2020-05-21","publish_status":"0","recid":"15187","relation_version_is_last":true,"title":["Regulation of KIF2A by Antitumor miR-451a Inhibits Cancer Cell Aggressiveness Features in Lung Squamous Cell  Carcinoma"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-09T01:57:32.661415+00:00"}