{"created":"2023-07-25T08:14:12.380204+00:00","id":15189,"links":{},"metadata":{"_buckets":{"deposit":"b0407b09-3c66-494c-a9ea-896cceddd47f"},"_deposit":{"created_by":18,"id":"15189","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"15189"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00015189","sets":["57:86"]},"author_link":["135855"],"item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2020-03-06","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2020-03-12"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Medical Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"In the present study, in order to elucidate the aggressive nature of lung squamous cell carcinoma (LUSQ), we investigated the oncogenic RNA networks regulated by antitumor microRNAs (miRNAs or miRs) in LUSQ cells. The analysis of our original miRNA expression signatures of human cancers revealed that microRNA‑150‑5p (miR‑150‑5p) was downregulated in various types of cancer, indicating that miR‑150‑5p acts as an antitumor miRNA by targeting several oncogenic genes. Thus, the aims of this study were to investigate the antitumor roles of miR‑150‑5p in LUSQ cells and to identify oncogenes regulated by miR‑150‑5p that are involved in the aggressive behavior of LUSQ. The downregulation of miR‑150‑5p was validated in clinical samples of LUSQ and cell lines (SK-MES‑1 and EBC‑1). The ectopic overexpression of miR‑150‑5p significantly suppressed cancer cell aggressiveness. Comprehensive gene expression analyses revealed that miR‑150‑5p regulated 9 genes in the LUSQ cells. Among these, matrix metalloproteinase 14 (MMP14) was found to be a direct target of miR‑150‑5p, as shown by luciferase reporter assay. The knockdown of MMP14 using siRNA against MMP14 (si-MMP14) significantly inhibited cancer cell migration and invasion. The overexpression of MMP14 was detected in clinical specimens of LUSQ by immunohistochemistry. On the whole, these findings suggest that the downregulation of miR‑150‑5p and the overexpression of MMP14 may be deeply involved in the pathogenesis of LUSQ.\n\nTakayuki Suetsugu, Keiichi Koshizuka, Naohiko Seki, Keiko Mizuno, Atsushi Okato, Takayuki Arai, Shunsuke Misono, Akifumi Uchida, Tomohiro Kumamoto, Hiromasa Inoue\nDownregulation of matrix metalloproteinase 14 by the antitumor miRNA, miR‑150‑5p, inhibits the aggressiveness of lung squamous cell carcinoma cells\nInternational Journal of Oncology 2018 52(3) 913-924\nhttps://doi.org/10.3892/ijo.2017.4232","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲総研第559号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"Spandidos Publications","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"カゴシマ ダイガク","subitem_publisher_language":"ja-Kana"}]},"item_5_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_value":"【指導教員:井上博雅】"}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2019-11-06"}]},"item_5_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"末次, 隆行","creatorNameLang":"ja"},{"creatorName":"スエツグ, タカユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Suetsugu, Takayuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-05"}],"displaytype":"detail","filename":"Diss_末次_隆行_ISK559_2019.pdf","filesize":[{"value":"917.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Diss_末次_隆行_ISK559_2019","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/15189/files/Diss_末次_隆行_ISK559_2019.pdf"},"version_id":"91b3372b-f93c-4e3f-9828-2bb90335b055"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-05"}],"displaytype":"detail","filename":"Abstract_末次_隆行_4517810179_2019.pdf","filesize":[{"value":"104.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Abstract_末次_隆行_4517810179_2019","objectType":"abstract","url":"https://ir.kagoshima-u.ac.jp/record/15189/files/Abstract_末次_隆行_4517810179_2019.pdf"},"version_id":"2151cfc4-fd01-49cc-8764-73972d638f8d"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-05-21"}],"displaytype":"detail","filename":"Result_Suetsugu_Takayuki_isk_559.pdf","filesize":[{"value":"219.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Result_Suetsugu_Takayuki_isk_559","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/15189/files/Result_Suetsugu_Takayuki_isk_559.pdf"},"version_id":"fbd707d5-9e6a-4ec9-9356-20ee05843145"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-05-21"}],"displaytype":"detail","filename":"Comments_Suetsugu_Takayuki_isk_559.pdf","filesize":[{"value":"101.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Comments_Suetsugu_Takayuki_isk_559","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/15189/files/Comments_Suetsugu_Takayuki_isk_559.pdf"},"version_id":"66c1a349-7838-4150-a4e4-4a00eb3dea68"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"microRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"lung squamous cell carcinoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"miR‑150‑5p","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"matrix metalloproteinase 14","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"antitumor","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Downregulation of matrix metalloproteinase 14 by the antitumor miRNA, miR-150-5p, inhibits the aggressiveness of lung squamous cell carcinoma cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Downregulation of matrix metalloproteinase 14 by the antitumor miRNA, miR-150-5p, inhibits the aggressiveness of lung squamous cell carcinoma cells","subitem_title_language":"en"},{"subitem_title":"癌抑制型マイクロRNA(miR-150-5p)はmatrix metalloproteinase 14 を制御して肺扁平上皮癌の増悪を抑制する","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"18","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-05-21"},"publish_date":"2020-05-21","publish_status":"0","recid":"15189","relation_version_is_last":true,"title":["Downregulation of matrix metalloproteinase 14 by the antitumor miRNA, miR-150-5p, inhibits the aggressiveness of lung squamous cell carcinoma cells"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-09T01:57:39.579049+00:00"}