{"created":"2023-07-25T08:14:14.589920+00:00","id":15239,"links":{},"metadata":{"_buckets":{"deposit":"23cd2a17-164b-4bac-91e7-71b70077e73f"},"_deposit":{"created_by":18,"id":"15239","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"15239"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00015239","sets":["57:86"]},"author_link":["136139"],"item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2020-04-02","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2020-04-20"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(歯学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Dental Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Nisin A is a bacteriocin produced by Lactococcus lactis and is widely used as a food preservative. Staphylococcus aureus has the BraRS-VraDE system that provides resistance against low concentrations of nisin A. BraRS is a two-component system that induces the expression of the ABC transporter VraDE. Previously, we isolated a highly nisin A-resistant strain with increased VraDE expression due to a mutation in braRS. In this study, we isolated S. aureus MW2 mutants with BraRS-VraDE-independent nisin A resistance. These mutants, designated SAN2 (S. aureus nisin resistant) and SAN469, had a mutation in pmtR, which encodes a transcriptional regulator responsible for the expression of the pmtABCD operon. As a result, these mutants exhibited increased expression of PmtABCD, a transporter responsible for the export of phenol-soluble modulin (PSM). Characterization of the mutants revealed that they have decreased susceptibility to human β-defensin-3 (hBD3) and LL37, which are innate immune factors. Additionally, these mutants showed higher hemolytic activity than the original MW2 strain. Furthermore, in a mouse bacteremia model, the SAN2 strain exhibited a lower survival rate than the original MW2 strain. These results indicate that the increased expression of pmtABCD due to a pmtR mutation is an alternative nisin A resistance mechanism that also affects virulence in S. aureus.\n\nMiki Kawada-Matsuo, Atsuko Watanabe, Kaoru Arii, Yuichi Oogai, Kazuyuki Noguchi, Shouichi Miyawaki, Tetsuya Hayashi, Hitoshi Komatsuzawa\nStaphylococcus aureus Virulence Affected by an Alternative Nisin A Resistance Mechanism\nApplied and Environmental Microbiology 2020\nDOI: 10.1128/AEM.02923-19","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲総研第561号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Microbiology","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学 ","subitem_publisher_language":"ja"},{"subitem_publisher":"カゴシマ ダイガク","subitem_publisher_language":"ja-Kana"}]},"item_5_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_value":"【指導教員:宮脇正一】"}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2020-03-04"}]},"item_5_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"渡邉, 温子","creatorNameLang":"ja"},{"creatorName":"ワタナベ, アツコ","creatorNameLang":"ja-Kana"},{"creatorName":"Watanabe, Atsuko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"136139","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-04-30"}],"displaytype":"detail","filename":"Diss_渡邉_温子_ISK561_2020.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Diss_渡邉_温子_ISK561_2020","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/15239/files/Diss_渡邉_温子_ISK561_2020.pdf"},"version_id":"2c6f65d0-6bfa-4316-bd01-c154161bec38"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-04-30"}],"displaytype":"detail","filename":"Abstract_渡邉_温子_4516800196_2019.pdf","filesize":[{"value":"228.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Abstract_渡邉_温子_4516800196_2019","objectType":"abstract","url":"https://ir.kagoshima-u.ac.jp/record/15239/files/Abstract_渡邉_温子_4516800196_2019.pdf"},"version_id":"38ab9649-d606-4be0-b46c-ac7e143f4212"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-07-08"}],"displaytype":"detail","filename":"Result_Watanabe_Atsuko_isk_561.pdf","filesize":[{"value":"988.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Result_Watanabe_Atsuko_isk_561","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/15239/files/Result_Watanabe_Atsuko_isk_561.pdf"},"version_id":"1b119b54-5024-4544-97f4-6f95e48b2be9"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-07-08"}],"displaytype":"detail","filename":"Comments_Watanabe_Atsuko_isk_561.pdf","filesize":[{"value":"541.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Comments_Watanabe_Atsuko_isk_561","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/15239/files/Comments_Watanabe_Atsuko_isk_561.pdf"},"version_id":"8332392d-4822-4c74-afd6-25b4ad9438aa"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"An alternative nisin A resistance mechanism affects virulence in Staphylococcus aureus","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"An alternative nisin A resistance mechanism affects virulence in Staphylococcus aureus","subitem_title_language":"en"},{"subitem_title":"ナイシンA作用により分離したナイシンA高度耐性MRSAのビルレンスに及ぼす影響","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"18","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-07-08"},"publish_date":"2020-07-08","publish_status":"0","recid":"15239","relation_version_is_last":true,"title":["An alternative nisin A resistance mechanism affects virulence in Staphylococcus aureus"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-09T01:57:46.025099+00:00"}