{"created":"2023-07-25T08:14:32.674233+00:00","id":15603,"links":{},"metadata":{"_buckets":{"deposit":"3edb539a-8ae4-4c80-958f-b91ac901f9ec"},"_deposit":{"created_by":18,"id":"15603","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"15603"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00015603","sets":["57:86"]},"author_link":["138133"],"control_number":"15603","item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2020-10-08","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2020-10-16"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Medical Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Background/Aim: 5-Aza-2-deoxycytidine (5-Aza-CdR) enhances the sensitivity to 5-fluorouracil (5-FU), but the molecular mechanism is not fully understood. The aim of this study was to investigate the molecular mechanism that enhances the sensitivity to 5-FU treated with 5-Aza-CdR via thymidine phosphorylase (TP). Materials and Methods: The sensitivity to drugs was determined on several cancer cell lines by the MTT assay. Protein and mRNA levels were examined by immunoblot and RT-PCR, respectively. Gene silencing, binding of Sp1 to DNA and methylation of DNA was performed by siRNA, ChIP assay and sodium bisulfate genomic sequencing, respectively. Results: Sp1-binding sites in the TP promoter were methylated in epidermoid carcinoma. 5-Aza-CdR demethylated Sp1-binding sites and enhanced sensitivity to 5-FU. Conclusion: Demethylation of Sp1-binding sites by 5-Aza-CdR was a key factor enhancing 5-FU sensitivity, which may enable more effective treatments for cancer patients with the combination of 5-Aza-CdR and 5-FU.\n\nYUKIHIKO NISHIZAWA, RYUJI IKEDA, MASATATSU YAMAMOTO, KOHICHI KAWAHARA, YOSHINARI SHINSATO, KENTARO MINAMI, MINA NITTA, HIDEYUKI TERAZONO, SHIN-ICHI AKIYAMA, TATSUHIKO FURUKAWA and YASUO TAKEDA\n5-Aza-2-deoxycytidine Enhances the Sensitivity of 5-Fluorouracil by Demethylation of the Thymidine Phosphorylase Promoter\nAnticancer Research, 2019, 39(8) p.4129-4136\nDOI: https://doi.org/10.21873/anticanres.13571","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"乙総論第40号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"International Institute of Anticancer Research","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"カゴシマ ダイガク","subitem_publisher_language":"ja-Kana"}]},"item_5_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_value":"【指導教員:武田泰生】"}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2020-08-05"}]},"item_5_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"西澤, 由紀彦","creatorNameLang":"ja"},{"creatorName":"ニシザワ, ユキヒコ","creatorNameLang":"ja-Kana"},{"creatorName":"Nishizawa, Yukihiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"138133","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-05-19"}],"displaytype":"detail","filename":"Diss_西澤_由紀彦_ISK40_2020.pdf","filesize":[{"value":"387.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Diss_西澤_由紀彦_ISK40_2020","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/15603/files/Diss_西澤_由紀彦_ISK40_2020.pdf"},"version_id":"8f47a0e3-ba6f-4328-880d-7c6dbcd72569"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-05-10"}],"displaytype":"detail","filename":"Abstract_西澤_由紀彦_TO_2020.pdf","filesize":[{"value":"242.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Abstract_西澤_由紀彦_TO_2020","objectType":"abstract","url":"https://ir.kagoshima-u.ac.jp/record/15603/files/Abstract_西澤_由紀彦_TO_2020.pdf"},"version_id":"0161ef65-5c17-499c-9936-3501160a0f1a"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-10-27"}],"displaytype":"detail","filename":"Result_Nishizawa_Yukihiko_isk_40.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Result_Nishizawa_Yukihiko_isk_40","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/15603/files/Result_Nishizawa_Yukihiko_isk_40.pdf"},"version_id":"ec6155b0-28fb-40f9-8320-ee1398c14182"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-10-27"}],"displaytype":"detail","filename":"Comments_Nishizawa_Yukihiko_isk_40.pdf","filesize":[{"value":"484.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Comments_Nishizawa_Yukihiko_isk_40","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/15603/files/Comments_Nishizawa_Yukihiko_isk_40.pdf"},"version_id":"8c1b8004-b217-452e-bc7e-fc26dea6b463"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"5-fluorouracil","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"5-aza-2-deoxycytidine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"thymidine phosphorylase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"methylation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"5-Aza-2-deoxycytidine enhances thesensitivity of 5-fluorouracil by demethylation of the thymidine phosphorylase promoter","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"5-Aza-2-deoxycytidine enhances thesensitivity of 5-fluorouracil by demethylation of the thymidine phosphorylase promoter","subitem_title_language":"en"},{"subitem_title":"5-Aza-2-deoxycytidineはthymidine phosphorylaseの脱メチル化を介して5-fluorouracilの感受性を高める","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"18","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-10-27"},"publish_date":"2020-10-27","publish_status":"0","recid":"15603","relation_version_is_last":true,"title":["5-Aza-2-deoxycytidine enhances thesensitivity of 5-fluorouracil by demethylation of the thymidine phosphorylase promoter"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-04-12T00:44:20.314432+00:00"}