@phdthesis{oai:ir.kagoshima-u.ac.jp:00015811,
 author = {和田, 真澄 and Wada, Masumi},
 month = {2021-09-07, 2021-09-07, 2021-04-09, 2021-04-09},
 note = {博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨, Aberrantly expressed microRNAs (miRNAs) disrupt intracellular RNA networks and contribute to malignant transformation of cancer cells. Utilizing the latest RNA-sequencing technology, wenewly created the miRNA expression signature of esophageal squamous cell carcinoma (ESCC). A total of 47 miRNAs were downregulated in ESCC tissues, and these miRNAs were candidates for antitumor miRNAs in ESCC cells. Analysis of the signature revealed that several passenger strands of miRNAs were significantly downregulated in ESCC, e.g., miR-28-3p, miR-30a-3p, miR-30c-3p, miR-133a-3b, miR-139-3p, miR-143-5p, and miR-145-3p. Recent studies indicate that some passenger strands of miRNAs closely involved in cancer pathogenesis. In this study, we focused on both strands of pre-miR-143, and investigated their antitumor roles and target oncogenes in ESCC. Ectopic expression of miR-143-5p and miR-143-3p significantly attenuated malignant phenotypes (e.g., proliferation, migration, and invasive abilities) in ESCC cell lines. We revealed that 6 genes (HN1, HMGA2, NETO2, STMN1, TCF3 and MET) were putative targets of miR-143-5p regulation, and one gene (KRT80) was a putative target of miR-143-3p regulation in ESCC cells. Our ESCC miRNA signature and analysis strategy provided important insights into the molecular pathogenesis of ESCC.

Masumi Wada, Yusuke Goto, Takako Tanaka, Reona Okada, Shogo Moriya, Tetsuya Idichi, Masahiro Noda, Ken Sasaki, Yoshiaki Kita, Hiroshi Kurahara, Kosei Maemura, Shoji Natsugoe & Naohiko Seki
RNA sequencing-based microRNA expression signature in esophageal squamous cell carcinoma: oncogenic targets by antitumor miR-143-5p and miR-143-3p regulation
Journal of Human Genetics volume 65, pages1019–1034 (2020)
https://doi.org/10.1038/s10038-020-0795-x},
 school = {鹿児島大学},
 title = {RNA sequencing-based microRNA expression signature in esophageal squamous cell carcinoma : oncogenic targets by antitumor miR-143-5p and miR-143-3p regulation},
 year = {},
 yomi = {ワダ, マスミ}
}