{"created":"2023-07-25T08:14:44.244619+00:00","id":15870,"links":{},"metadata":{"_buckets":{"deposit":"ed62dd74-079c-4647-bbd3-30ae0b351123"},"_deposit":{"created_by":18,"id":"15870","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"15870"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00015870","sets":["57:86"]},"author_link":["139476"],"item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2021-05-11","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2021-05-17"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Medical Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Our previous studies of the microRNA (miRNA) expression signature in clear cell renal cell carcinoma (ccRCC) indicated that miRNA-1274a (miR-1274a) was significantly upregulated in clinical specimens, suggesting that miR-1274a may act as an oncogenic miRNA in ccRCC. The aim of this study was to investigate the functional roles of miR-1274a and identify downstream tumor-suppressive targets regulated by miR‑1274a in ccRCC cells. Functional studies of miR-1274a were carried out by anti-miRNA to investigate cell proliferation and apoptosis using the A498, ACHN and Caki1 ccRCC cell lines. Suppression of miR-1274a significantly inhibited cancer cell proliferation and induced apoptosis in the ccRCC cells. Gene expression data combined with in silico analysis and luciferase reporter assays demonstrated that bone morphogenetic protein receptor type 1B (BMPR1B) was directly regulated by miR-1274a. Moreover, TCGA database as well as immunohistochemistry demonstrated low expression of BMPR1B in ccRCC clinical specimens compared to that in normal kidney tissues. We conclude that loss of oncogenic miR-1274a reduced cancer cell proliferation and induced apoptosis in ccRCC through targeting BMPR1B. Our data revealing molecular pathways and a target gene regulated by oncogenic miR-1274a provide new insight into the potential mechanisms of ccRCC oncogenesis.\n\nHirofumi Yoshino, Tomokazu Yonezawa, Masaya Yonemori, Kazutaka Miyamoto, Takashi Sakaguchi, Satoru Sugita, Youichi Osako, Shuichi Tatarano, Masayuki Nakagawa, Hideki Enokida\nDownregulation of microRNA-1274a induces cell apoptosis through regulation of BMPR1B in clear cell renal cell carcinoma\nOncology Reports 2018 39(1) p.173-181\nhttps://doi.org/10.3892/or.2017.6098","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲総研第605号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"Spandidos Publications","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"カゴシマ ダイガク","subitem_publisher_language":"ja-Kana"}]},"item_5_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_value":"【指導教員:榎田英樹】"}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2020-12-02"}]},"item_5_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"米澤, 智一","creatorNameLang":"ja"},{"creatorName":"ヨネザワ, トモカズ","creatorNameLang":"ja-Kana"},{"creatorName":"Yonezawa, Tomokazu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"139476","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-06-01"}],"displaytype":"detail","filename":"Diss_米澤_智一_ISK605_2021.pdf","filesize":[{"value":"638.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Diss_米澤_智一_ISK605_2021","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/15870/files/Diss_米澤_智一_ISK605_2021.pdf"},"version_id":"a3268119-e044-41f8-af55-ae960a0f6d22"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-09"}],"displaytype":"detail","filename":"Abstract_米澤_智一_4510810239_2020.pdf","filesize":[{"value":"54.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Abstract_米澤_智一_4510810239_2020","objectType":"abstract","url":"https://ir.kagoshima-u.ac.jp/record/15870/files/Abstract_米澤_智一_4510810239_2020.pdf"},"version_id":"66ff12c8-cff5-42e7-bfc1-d3e2154b69b2"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-05-26"}],"displaytype":"detail","filename":"Result_Yonezawa_Tomokazu_isk_605.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Result_Yonezawa_Tomokazu_isk_605","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/15870/files/Result_Yonezawa_Tomokazu_isk_605.pdf"},"version_id":"e2e8cf64-a3de-45f1-8077-e960ef45404c"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-05-26"}],"displaytype":"detail","filename":"Comments_Yonezawa_Tomokazu_isk_605.pdf","filesize":[{"value":"524.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Comments_Yonezawa_Tomokazu_isk_605","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/15870/files/Comments_Yonezawa_Tomokazu_isk_605.pdf"},"version_id":"1fd7adb3-bfa6-4c3c-9e60-cd59e11f0c63"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"microRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"miR-1274a","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"renal cell carcinoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"apoptosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"upregulated miRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Downregulation of microRNA-1274a induces cell apoptosis through regulation of BMPR1B in clear cell renal cell carcinoma","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Downregulation of microRNA-1274a induces cell apoptosis through regulation of BMPR1B in clear cell renal cell carcinoma","subitem_title_language":"en"},{"subitem_title":"淡明腎細胞癌において、miRNA-1274aを抑制することによりBMPR1Bの発現が回復し、癌細胞のアポトーシスが誘導される","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"18","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-05-26"},"publish_date":"2021-05-26","publish_status":"0","recid":"15870","relation_version_is_last":true,"title":["Downregulation of microRNA-1274a induces cell apoptosis through regulation of BMPR1B in clear cell renal cell carcinoma"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-09T02:00:05.372108+00:00"}