{"created":"2023-07-25T08:14:45.425020+00:00","id":15896,"links":{},"metadata":{"_buckets":{"deposit":"cadbbeda-a268-4cdb-9810-80cea826b2ba"},"_deposit":{"created_by":18,"id":"15896","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"15896"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00015896","sets":["57:86"]},"author_link":["139638"],"item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2021-08-05","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2021-08-11"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Medical Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Purkinje cell protein 4/peptide 19 (PCP4/PEP19) is 7.6 kDa peptide originally found in Purkinje cells. PCP4/PEP19 is a differentiation maker of Purkinje cells, where it functions as an antiapoptotic factor. Cerebral neuronal cells also express PCP4/PEP19, which may be related to neuronal cell survival. However, evidence suggests that PCP4/PEP19 may also be involved in neuronal differentiation. Here, we investigated the effects of PCP4/PEP19 expression on neuronal differentiation by analyzing neurite outgrowth, and expression of neuronal differentiation markers in cultured human neuroblastoma M17 cells. When PCP4/PEP19 expression was reduced by siRNA-mediated knockdown, neurite outgrowth was significantly increased. Among many differentiation markers tested, expression of NeuroD1 was increased, while that of Ascl1 was decreased upon PCP4/PEP19 knockdown. Furthermore, luciferase reporter assays revealed that PCP4/PEP19 knockdown upregulated NeuroD1 and downregulated Ascl1 expression, at the transcriptional level. These results suggest a new function of PCP4/PEP19, which suppresses neurite outgrowth and neuronal differentiation through the regulation of NeuroD1 and Ascl1 expression in M17 cells. Furthermore, immunohistochemical studies showed that PCP4/PEP19 localizes in the nuclei of human neuroblastoma cells. Therefore, PCP4/PEP19 may also be an intranuclear negative regulator of neuronal differentiation and may thus be a potential therapeutic target to promote cellular differentiation in human neuroblastoma.\n\nIkumi Kitazono, Taiji Hamada, Takuya Yoshimura, Mari Kirishima, Seiya Yokoyama, Toshiaki Akahane & Akihide Tanimoto\nPCP4/PEP19 downregulates neurite outgrowth via transcriptional regulation of Ascl1 and NeuroD1 expression in human neuroblastoma M17 cells\nLaboratory Investigation 100, p.1551–1563 (2020)\nhttps://doi.org/10.1038/s41374-020-0462-z","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"乙総論第41号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"nature","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"カゴシマ ダイガク","subitem_publisher_language":"ja-Kana"}]},"item_5_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_value":"【指導教員:谷本昭英】"}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2021-04-07"}]},"item_5_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"北薗, 育美","creatorNameLang":"ja"},{"creatorName":"キタゾノ, イクミ","creatorNameLang":"ja-Kana"},{"creatorName":"Kitazono, Ikumi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-08-30"}],"displaytype":"detail","filename":"Diss_北薗_育美_ISK41_2021.pdf","filesize":[{"value":"2.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Diss_北薗_育美_ISK41_2021","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/15896/files/Diss_北薗_育美_ISK41_2021.pdf"},"version_id":"fb64c8d6-bd55-422f-b54a-f3ce71c2d337"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-08-30"}],"displaytype":"detail","filename":"Abstract_北薗_育美_TO_2021.pdf","filesize":[{"value":"107.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Abstract_北薗_育美_TO_2021","objectType":"abstract","url":"https://ir.kagoshima-u.ac.jp/record/15896/files/Abstract_北薗_育美_TO_2021.pdf"},"version_id":"714b382c-08ad-4e1f-abc1-62758ac1427a"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-08-30"}],"displaytype":"detail","filename":"Result_kitazono_ikumi_isk41.pdf","filesize":[{"value":"817.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Result_kitazono_ikumi_isk41","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/15896/files/Result_kitazono_ikumi_isk41.pdf"},"version_id":"6f31d340-cde1-418b-b3a1-ec4122ffa999"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-08-30"}],"displaytype":"detail","filename":"Comments_kitazono_ikumi_isk_41.pdf","filesize":[{"value":"421.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Comments_kitazono_ikumi_isk_41","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/15896/files/Comments_kitazono_ikumi_isk_41.pdf"},"version_id":"5f8fd465-1dbc-43fc-a41a-f1e6ba7af051"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"PCP4/PEP19 downregulates neurite outgrowth via transcriptional regulation of Ascl1 and NeuroD1 expression in human neuroblastoma M17 cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"PCP4/PEP19 downregulates neurite outgrowth via transcriptional regulation of Ascl1 and NeuroD1 expression in human neuroblastoma M17 cells","subitem_title_language":"en"},{"subitem_title":"PCP4/PEP19は、ヒト神経芽腫M17において、 Ascl1 および NeuroD1 発現の転写制御を介して神経突起の伸長を制御する。","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"18","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-08-30"},"publish_date":"2021-08-30","publish_status":"0","recid":"15896","relation_version_is_last":true,"title":["PCP4/PEP19 downregulates neurite outgrowth via transcriptional regulation of Ascl1 and NeuroD1 expression in human neuroblastoma M17 cells"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-09T02:05:11.364624+00:00"}