{"created":"2023-07-25T08:14:53.838841+00:00","id":16083,"links":{},"metadata":{"_buckets":{"deposit":"fa2d219f-3313-4684-b9d6-f1ece3d75567"},"_deposit":{"created_by":18,"id":"16083","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"16083"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00016083","sets":["57:86"]},"author_link":["140620"],"item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2022-02-07","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2022-02-17"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Medical Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Mitochondrial functional abnormalities or quantitative decreases are considered to be one of the most plausible pathogenic mechanisms of Parkinson’s disease (PD). Thus, mitochondrial complex inhibitors are often used for the development of experimental PD. In this study, we used rotenone to create in vitro cell models of PD, then used these models to investigate the effects of 1,5-anhydro-D-fructose (1,5-AF), a monosaccharide with protective effects against a range of cytotoxic substances. Subsequently, we investigated the possible mechanisms of these protective effects in PC12 cells. The protection of 1,5-AF against rotenone-induced cytotoxicity was confirmed by increased cell viability and longer dendritic lengths in PC12 and primary neuronal cells. Furthermore, in rotenone-treated PC12 cells, 1,5-AF upregulated peroxisome proliferator-activated receptor-γ coactivator 1α (PGC-1α) expression and enhanced its deacetylation, while increasing AMP-activated protein kinase (AMPK) phosphorylation. 1,5-AF treatment also increased mitochondrial activity in these cells. Moreover, PGC-1α silencing inhibited the cytoprotective and mitochondrial biogenic effects of 1,5-AF in PC12 cells. Therefore, 1,5-AF may activate PGC-1α through AMPK activation, thus leading to mitochondrial biogenic and cytoprotective effects. Together, our results suggest that 1,5-AF has therapeutic potential for development as a treatment for PD.\n\nYuki Kasamo, Kiyoshi Kikuchi, Munekazu Yamakuchi, Shotaro Otsuka, \nSeiya Takada, Yuki Kambe, Takashi Ito, Ko-ichi Kawahara, Kazunori Arita,  Koji Yoshimoto and Ikuro Maruyama\n1,5-Anhydro-D-fructose Protects against Rotenone-Induced Neuronal Damage In Vitro through Mitochondrial Biogenesis\nInternational Journal of Molecular Sciences 2021, 22, 9941\nhttps://doi.org/10.3390/ijms22189941","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲総研第631号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"カゴシマ ダイガク","subitem_publisher_language":"ja-Kana"}]},"item_5_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_value":"【指導教員:吉本幸司】"}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2021-11-04"}]},"item_5_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"笠毛, 友揮","creatorNameLang":"ja"},{"creatorName":"カサモ, ユウキ","creatorNameLang":"ja-Kana"},{"creatorName":"Kasamo, Yuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-06-21"}],"displaytype":"detail","filename":"Diss_笠毛_友揮_ISK631_2021.pdf","filesize":[{"value":"4.3 MB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"Diss_笠毛_友揮_ISK631_2021","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/16083/files/Diss_笠毛_友揮_ISK631_2021.pdf"},"version_id":"5d1e38fd-bf4b-453a-9b50-f59aba9188fa"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-06-20"}],"displaytype":"detail","filename":"Abstract_笠毛_友揮_4513810063_2021.pdf","filesize":[{"value":"181.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Abstract_笠毛_友揮_4513810063_2021","objectType":"abstract","url":"https://ir.kagoshima-u.ac.jp/record/16083/files/Abstract_笠毛_友揮_4513810063_2021.pdf"},"version_id":"56539802-d13d-4d74-8dce-d0c56d6de2eb"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-06-06"}],"displaytype":"detail","filename":"Result_Kasamo_Yuki_isk_631.pdf","filesize":[{"value":"131.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Result_Kasamo_Yuki_isk_631","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/16083/files/Result_Kasamo_Yuki_isk_631.pdf"},"version_id":"9d15cf7e-0d06-44c0-b6a4-2b408ec02674"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-06-06"}],"displaytype":"detail","filename":"Comments_Kasamo_Yuki_isk_631.pdf","filesize":[{"value":"51.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Comments_Kasamo_Yuki_isk_631","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/16083/files/Comments_Kasamo_Yuki_isk_631.pdf"},"version_id":"4267b08a-68db-4bf8-8198-80e5ca58d731"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"1,5-AF","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"1,5-AG","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"metformin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Parkinson’s disease","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"parkinsonism","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"AMPK","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PGC-1α","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mitochondria","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mitochondrial biogenesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"rotenone","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"1,5-Anhydro-D-Fructose Protects against Rotenone-Induced Neuronal Damage In Vitro Through Mitochondrial Biogenesis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"1,5-Anhydro-D-Fructose Protects against Rotenone-Induced Neuronal Damage In Vitro Through Mitochondrial Biogenesis","subitem_title_language":"en"},{"subitem_title":"1,5-アンヒドロ-D-フラクトースはミトコンドリアの生成を介して、In vitroでのロテノンによる神経細胞の損傷を防ぐ","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"18","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-05-23"},"publish_date":"2022-05-23","publish_status":"0","recid":"16083","relation_version_is_last":true,"title":["1,5-Anhydro-D-Fructose Protects against Rotenone-Induced Neuronal Damage In Vitro Through Mitochondrial Biogenesis"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-09T02:05:59.088789+00:00"}