@phdthesis{oai:ir.kagoshima-u.ac.jp:00016094,
 author = {勝俣, 環 and Katsumata, Tamaki},
 month = {2022-12-21, 2022-07-27, 2022-05-27, 2022-05-27},
 note = {博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨, Streptococcus mutans, a cariogenic pathogen, adheres to the tooth surface and forms a biofilm. Bacterial cell surface proteins are associated with adherence to substrates. Sortase A (SrtA) mediates the localization of proteins with an LPXTG motif-containing proteins to the cell surface by covalent binding to peptidoglycan. In S. mutans UA159, six SrtA-dependent proteins, SpaP, WapA, WapE, DexA, FruA, and GbpC, were identified. Although some of these proteins were characterized, a comprehensive analysis of the six proteins has not been reported. In this study, we constructed mutants deficient in each of these proteins and the SrtA-deficient mutant. The SrtA-deficient mutant showed drastically decreased binding to salivary components, biofilm formation, bacterial coaggregation activity, hydrophobicity, and cellular matrix binding (collagen type I, fibronectin, and laminin). The SpaP-deficient mutant showed significantly reduced binding to salivary components and partially increased coaggregation with Porphyromonas gingivalis, and decreased hydrophobicity, and collagen binding. The WapA-deficient mutant showed slightly decreased coaggregation with Fusobacterium nucleatum. Although the SrtA-deficient mutant showed drastically altered phenotypes, all SrtA-dependent protein-deficient mutants, except the SpaP-deficient mutant, did not show considerable alterations in binding to salivary components. These results indicate that the six proteins may coordinately contribute to these activities. In addition, using genomic data of 125 S. mutans strains, the amino acid sequences of each surface protein were compared and many variations were found among strains, which may affect the phenotype of cell surface proteins in S. mutans.

This is the peer reviewed version of the following article:
Tamaki Katsumata, Mi Nguyen-Tra Le, Miki Kawada-Matsuo, Yuri Taniguchi, Kazuhisa Ouhara, Yuichi Oogai, Masanobu Nakata, Noriyoshi Mizuno, Yoshihiro Nishitani, Hitoshi Komatsuzawa
Comprehensive characterization of sortase A-dependent surface proteins in Streptococcus mutans
Microbiol Immunol. 2022;66:145–156,
which has been published in final form at https://doi.org/10.1111/1348-0421.12958. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.},
 school = {鹿児島大学},
 title = {Comprehensive characterization of sortase A-dependent surface proteins in Streptococcus mutans},
 year = {},
 yomi = {カツマタ, タマキ}
}