{"created":"2023-07-25T08:14:56.177376+00:00","id":16130,"links":{},"metadata":{"_buckets":{"deposit":"95787cb4-1e41-4f11-a05c-b305c74c3c10"},"_deposit":{"created_by":18,"id":"16130","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"16130"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00016130","sets":["57:86"]},"author_link":["140742"],"item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2022-05-17","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2022-05-30"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Medical Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Lung cancer constitutes a threat to human health. BHLHE41 plays important roles in circadian rhythm and cell differentiation as a negative regulatory transcription factor. This study investigates the role of BHLHE41 in lung cancer progression. We analyzed BHLHE41 function via in silico and immunohistochemical studies of 177 surgically resected non-small cell lung cancer (NSCLC) samples and 18 early lung squamous cell carcinoma (LUSC) cases. We also examined doxycycline (DOX)-inducible BHLHE41-expressing A549 and H2030 adenocarcinoma cells. BHLHE41 expression was higher in normal lung than in lung adenocarcinoma (LUAD) tissues and was associated with better prognosis for the overall survival (OS) of patients. In total, 15 of 132 LUAD tissues expressed BHLHE41 in normal lung epithelial cells. Staining was mainly observed in adenocarcinoma in situ and the lepidic growth part of invasive cancer tissue. BHLHE41 expression constituted a favorable prognostic factor for OS (p = 0.049) and cause-specific survival (p = 0.042) in patients with LUAD. During early LUSC, 7 of 18 cases expressed BHLHE41, and this expression was inversely correlated with the depth of invasion. DOX suppressed cell proliferation and increased the autophagy protein LC3, while chloroquine enhanced LC3 accumulation and suppressed cell death. In a xenograft model, DOX suppressed tumor growth. Our results indicate that BHLHE41 expression prevents early lung tumor malignant progression by inducing autophagic cell death in NSCLC.\n\nToshiyuki Nagata, Kentaro Minami, Masatatsu Yamamoto, Tsubasa Hiraki,  Masashi Idogawa, Katsumi Fujimoto, Shun Kageyama, Kazuhiro Tabata,  Kohichi Kawahara, Kazuhiro Ueda, Ryuji Ikeda, Yukio Kato, Masaaki  Komatsu, Akihide Tanimoto, Tatsuhiko Furukawa and Masami Sato\nBHLHE41/DEC2 Expression Induces Autophagic Cell Death in Lung Cancer Cells and Is Associated with Favorable Prognosis for Patients with Lung Adenocarcinoma\nInternational Journal of Molecular Sciences 2021, 22, 11509\nhttps://doi.org/10.3390/ijms222111509","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲総研第658号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"カゴシマ ダイガク","subitem_publisher_language":"ja-Kana"}]},"item_5_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_value":"【指導教員:佐藤雅美】"}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2022-03-02"}]},"item_5_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"永田, 俊行","creatorNameLang":"ja"},{"creatorName":"ナガタ, トシユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Nagata, Toshiyuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-06-22"}],"displaytype":"detail","filename":"Diss_永田_俊行_ISK658_2022.pdf","filesize":[{"value":"7.4 MB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"Diss_永田_俊行_ISK658_2022","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/16130/files/Diss_永田_俊行_ISK658_2022.pdf"},"version_id":"9b3d9965-ab28-4be4-b3f8-93c061e69e1a"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-06-14"}],"displaytype":"detail","filename":"Abstract_永田_俊行_4511810126_2022.pdf","filesize":[{"value":"24.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Abstract_永田_俊行_4511810126_2022","objectType":"abstract","url":"https://ir.kagoshima-u.ac.jp/record/16130/files/Abstract_永田_俊行_4511810126_2022.pdf"},"version_id":"db9d797c-5afe-4263-a5c3-651f86309fae"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-06-13"}],"displaytype":"detail","filename":"Result_Nagata_toshiyuki_isk_658.pdf","filesize":[{"value":"120.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Result_Nagata_toshiyuki_isk_658","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/16130/files/Result_Nagata_toshiyuki_isk_658.pdf"},"version_id":"3cd93f7f-3932-4f06-982e-483c60a3df8d"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-06-13"}],"displaytype":"detail","filename":"Comments_Nagata_toshiyuki_isk_658.pdf","filesize":[{"value":"62.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Comments_Nagata_toshiyuki_isk_658","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/16130/files/Comments_Nagata_toshiyuki_isk_658.pdf"},"version_id":"bf695b5b-cc07-4328-9b6d-283bcd6b78fd"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"non-small cell lung cancer","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"BHLHE41","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"autophagic cell death","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"non-invasiveness","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"BHLHE41/DEC2 Expression Induces Autophagic Cell Death in Lung Cancer Cells and is Associated with Favorable Prognosis for Patients with Lung Adenocarcinoma","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"BHLHE41/DEC2 Expression Induces Autophagic Cell Death in Lung Cancer Cells and is Associated with Favorable Prognosis for Patients with Lung Adenocarcinoma","subitem_title_language":"en"},{"subitem_title":"BHLHE41/DEC2の発現は肺癌細胞の自食細胞死を誘導し、肺腺癌患者の良好な予後と関連している","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"18","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-06-13"},"publish_date":"2022-06-13","publish_status":"0","recid":"16130","relation_version_is_last":true,"title":["BHLHE41/DEC2 Expression Induces Autophagic Cell Death in Lung Cancer Cells and is Associated with Favorable Prognosis for Patients with Lung Adenocarcinoma"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-09T02:07:19.174168+00:00"}