{"created":"2023-07-25T08:14:56.220464+00:00","id":16131,"links":{},"metadata":{"_buckets":{"deposit":"d05a0196-10e4-4818-87c6-755eb2f5b7ad"},"_deposit":{"created_by":18,"id":"16131","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"16131"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00016131","sets":["57:86"]},"author_link":["140745"],"control_number":"16131","item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2022-05-17","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2022-05-30"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Medical Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Drug resistance is a major problem in treatment with nelarabine, and its resolution requires elucidation of the underlying mechanisms. We established two nelarabine-resistant subclones of the human T-cell lymphoblastic leukemia cell line CCRF-CEM. The resistant subclones showed changes in the expression of several genes related to nelarabine intracellular activation and inhibition of apoptosis. Activation of the Akt protein upon nelarabine treatment was observed in both subclones. The combination treatment with nelarabine and PI3K/Akt inhibitors was shown to inhibit cell growth. Cross-resistance was observed with ara-C and not with vincristine, daunorubicin, or etoposide treatment. Thus, changes in the expression of cellular activation-related genes, inhibition of apoptosis, and induction of Akt may be involved in the development of nelarabine resistance in the CCRF-CEM cell model. The use of different classes of chemotherapeutic agents and combination therapy with PI3K/Akt pathway inhibitors may be used to overcome resistance to nelarabine.\n\nAlmitra Rindiarti, Yasuhiro Okamoto, Shunsuke Nakagawa, Junko Hirose, Yuichi Kodama, Takuro Nishikawa, Yoshifumi Kawano\nChanges in intracellular activation-related gene expression and induction of Akt contribute to acquired resistance toward nelarabine in CCRF-CEM cell line\nLeukemia & Lymphoma v.63(2) P.404-415 (2022)\n\nThis is an Accepted Manuscript of an article published by Taylor & Francis in Leukemia & Lymphoma on 26 Jan 2022,\navailable at: https://doi.org/10.1080/10428194.2021.1992617","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲総研第659号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"Taylor & Francis","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"カゴシマ ダイガク","subitem_publisher_language":"ja-Kana"}]},"item_5_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_value":"【指導教員:岡本康裕】"}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2022-03-02"}]},"item_5_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Rindiarti, Almitra","creatorNameLang":"en"},{"creatorName":"リンディアルティ, アルミトラ","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"140745","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-01-27"}],"displaytype":"detail","filename":"Diss_アルミトラ_リンディアルティ_ISK659_2022.pdf","filesize":[{"value":"3.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Diss_アルミトラ_リンディアルティ_ISK659_2022","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/16131/files/Diss_アルミトラ_リンディアルティ_ISK659_2022.pdf"},"version_id":"c5a45270-77b2-464c-be3a-aa02179b4aa3"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-06-14"}],"displaytype":"detail","filename":"Abstract_アルミトラ_リンディアルティ_4516800249_2022.pdf","filesize":[{"value":"359.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Abstract_アルミトラ_リンディアルティ_4516800249_2022","objectType":"abstract","url":"https://ir.kagoshima-u.ac.jp/record/16131/files/Abstract_アルミトラ_リンディアルティ_4516800249_2022.pdf"},"version_id":"0c446349-5e25-4de6-8d65-9f82e8a18097"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-06-13"}],"displaytype":"detail","filename":"Result_Almitra_Rindiarti_isk_659.pdf","filesize":[{"value":"115.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Result_Almitra_Rindiarti_isk_659","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/16131/files/Result_Almitra_Rindiarti_isk_659.pdf"},"version_id":"45e44772-ca4c-4be3-8b5f-5a35dce94187"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-06-13"}],"displaytype":"detail","filename":"Comments_Almitra_Rindiarti_isk_659 .pdf","filesize":[{"value":"52.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Comments_Almitra_Rindiarti_isk_659","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/16131/files/Comments_Almitra_Rindiarti_isk_659 .pdf"},"version_id":"480ada3b-9d76-4d53-95cf-6cb51db2de93"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"T-cell acute lymphoblastic leukemia","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"drug resistance","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"nelarabine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"combination therapy","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PI3K/Akt pathway","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Changes in intracellular activation-related gene expression and induction of Akt contribute to acquired resistance towards nelarabine in CCRF-CEM cell line","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Changes in intracellular activation-related gene expression and induction of Akt contribute to acquired resistance towards nelarabine in CCRF-CEM cell line","subitem_title_language":"en"},{"subitem_title":"CCRF-CEM細胞株における細胞内活性化関連遺伝子発現の変化とAktの誘導がネララビンに対する獲得抵抗性に寄与している","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"18","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-06-13"},"publish_date":"2022-06-13","publish_status":"0","recid":"16131","relation_version_is_last":true,"title":["Changes in intracellular activation-related gene expression and induction of Akt contribute to acquired resistance towards nelarabine in CCRF-CEM cell line"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-11T00:50:41.759950+00:00"}