@phdthesis{oai:ir.kagoshima-u.ac.jp:00016135,
 author = {赤羽, 俊章 and Akahane, Toshiaki},
 month = {2022-08-05, 2022-06-14, 2022-06-13, 2022-06-13},
 note = {博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨, Background: Genomic examination of cytology specimens is often performed on cell blocks or conventional smears rather than on liquid-based cytology (LBC) specimens. Since LBC specimens preserve high-quality DNA, cancer genome profiling using next-generation sequencing (NGS) is also attainable from residual LBC specimens. One of the advantages of using LBC specimens for NGS is that it allows direct extraction of DNA from residual specimens, avoiding a sacrifice of smear slides and minimizing genomic profiling processing time.
Methods: Endometrial LBC specimens were subjected to NGS analysis to validate the practicality of rapid cancer genomic profiling in a pathology laboratory. The extracted DNA was subjected to NGS using a customized cancer gene panel comprising 56 genes and 17 microsatellite regions. The workflow strategy was defined, and the processing time estimated for specimen sampling, cell counting, NGS run, and genome profiling.
Results: NGS analysis of most LBC specimens revealed somatic mutations, tumor mutation burden, and microsatellite instability, which were almost identical to those obtained from formalin-fixed paraffin-embedded tissues. The processing time for direct NGS analysis and cancer genomic profiling of the residual LBC specimens was approximately 5 days.
Conclusion: The residual LBC specimens collected using endometrial cytology were verified to carry a high tumor fraction for NGS analysis and could serve as an alternate source for rapid molecular classification and diagnosis of endometrial cancers, as a routine process in a pathology laboratory.

This is the peer reviewed version of the following article:
Toshiaki Akahane, Ikumi Kitazono, Yusuke Kobayashi, Yukari Nishida-Kirita, Tomomi Yamaguchi, Shintaro Yanazume, Kazuhiro Tabata, Hiroaki Kobayashi, Akihide Tanimoto
Direct next-generation sequencing analysis using endometrialliquid-based cytology specimens for rapid cancer genomicprofiling
Diagnostic Cytopathology.2021;49:1078–1085,
which has been published in final form at 
https://doi.org/10.1002/dc.24841. 
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 school = {鹿児島大学},
 title = {Direct next-generation sequencing analysis using endometrial liquid-based cytology specimens for rapid cancer genomic profiling},
 year = {},
 yomi = {アカハネ, トシアキ}
}