{"created":"2023-07-25T08:14:56.404416+00:00","id":16135,"links":{},"metadata":{"_buckets":{"deposit":"29b1d969-e117-4ada-83c9-35061224c37a"},"_deposit":{"created_by":18,"id":"16135","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"16135"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00016135","sets":["57:86"]},"author_link":["140755"],"item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2022-05-17","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2022-05-30"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Medical Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Background: Genomic examination of cytology specimens is often performed on cell blocks or conventional smears rather than on liquid-based cytology (LBC) specimens. Since LBC specimens preserve high-quality DNA, cancer genome profiling using next-generation sequencing (NGS) is also attainable from residual LBC specimens. One of the advantages of using LBC specimens for NGS is that it allows direct extraction of DNA from residual specimens, avoiding a sacrifice of smear slides and minimizing genomic profiling processing time.\nMethods: Endometrial LBC specimens were subjected to NGS analysis to validate the practicality of rapid cancer genomic profiling in a pathology laboratory. The extracted DNA was subjected to NGS using a customized cancer gene panel comprising 56 genes and 17 microsatellite regions. The workflow strategy was defined, and the processing time estimated for specimen sampling, cell counting, NGS run, and genome profiling.\nResults: NGS analysis of most LBC specimens revealed somatic mutations, tumor mutation burden, and microsatellite instability, which were almost identical to those obtained from formalin-fixed paraffin-embedded tissues. The processing time for direct NGS analysis and cancer genomic profiling of the residual LBC specimens was approximately 5 days.\nConclusion: The residual LBC specimens collected using endometrial cytology were verified to carry a high tumor fraction for NGS analysis and could serve as an alternate source for rapid molecular classification and diagnosis of endometrial cancers, as a routine process in a pathology laboratory.\n\nThis is the peer reviewed version of the following article:\nToshiaki Akahane, Ikumi Kitazono, Yusuke Kobayashi, Yukari Nishida-Kirita, Tomomi Yamaguchi, Shintaro Yanazume, Kazuhiro Tabata, Hiroaki Kobayashi, Akihide Tanimoto\nDirect next-generation sequencing analysis using endometrialliquid-based cytology specimens for rapid cancer genomicprofiling\nDiagnostic Cytopathology.2021;49:1078–1085,\nwhich has been published in final form at \nhttps://doi.org/10.1002/dc.24841. \nThis article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"乙総論第45号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"Wiley","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"カゴシマ ダイガク","subitem_publisher_language":"ja-Kana"}]},"item_5_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_value":"【指導教員:谷本昭英】"}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2022-01-05"}]},"item_5_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"赤羽, 俊章","creatorNameLang":"ja"},{"creatorName":"アカハネ, トシアキ","creatorNameLang":"ja-Kana"},{"creatorName":"Akahane, Toshiaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"140755","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-08-05"}],"displaytype":"detail","filename":"Diss_赤羽_俊章_ISK45_2022.pdf","filesize":[{"value":"390.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Diss_赤羽_俊章_ISK45_2022","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/16135/files/Diss_赤羽_俊章_ISK45_2022.pdf"},"version_id":"d3b09176-739b-4d6f-a686-3ca40f93ada6"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-06-14"}],"displaytype":"detail","filename":"Abstract_赤羽_俊章_TO_2021.pdf","filesize":[{"value":"74.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Abstract_赤羽_俊章_TO_2021","objectType":"abstract","url":"https://ir.kagoshima-u.ac.jp/record/16135/files/Abstract_赤羽_俊章_TO_2021.pdf"},"version_id":"27affc35-8d90-4b8c-a8df-a2f9d923aa26"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-06-13"}],"displaytype":"detail","filename":"Result_Akahane_toshiaki_isk_45.pdf","filesize":[{"value":"108.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Result_Akahane_toshiaki_isk_45","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/16135/files/Result_Akahane_toshiaki_isk_45.pdf"},"version_id":"c1c7a933-1fcc-42b9-b1a6-eca69292061d"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-06-13"}],"displaytype":"detail","filename":"Comments_Akahane_toshiaki_isk_45.pdf","filesize":[{"value":"57.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Comments_Akahane_toshiaki_isk_45","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/16135/files/Comments_Akahane_toshiaki_isk_45.pdf"},"version_id":"1143fd7f-bf84-4a05-ad10-74b36092a649"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cancer panel","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"endometrial cancer","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"liquid-based cytology","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"next-generation sequencing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"rapidgenomic profiling","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Direct next-generation sequencing analysis using endometrial liquid-based cytology specimens for rapid cancer genomic profiling","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Direct next-generation sequencing analysis using endometrial liquid-based cytology specimens for rapid cancer genomic profiling","subitem_title_language":"en"},{"subitem_title":"子宮内膜液状化細胞診検体を用いたダイレクトシークエンスによる迅速がんゲノムプロファイリング","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"18","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-06-13"},"publish_date":"2022-06-13","publish_status":"0","recid":"16135","relation_version_is_last":true,"title":["Direct next-generation sequencing analysis using endometrial liquid-based cytology specimens for rapid cancer genomic profiling"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-09T02:07:35.059558+00:00"}