{"created":"2023-07-25T08:15:02.071188+00:00","id":16255,"links":{},"metadata":{"_buckets":{"deposit":"f0bbab79-a6c5-4b3b-bc99-a85e9762b932"},"_deposit":{"created_by":18,"id":"16255","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"16255"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00016255","sets":["57:86"]},"author_link":["141341"],"item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2022-10-07","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2022-10-18"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(歯学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Dental Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Clinically, early-stage oral cancers are difficult to distinguish from oral potentially malignant disorders (OPMDs), and invasive tissue biopsy should be performed to determine a treatment strategy. Previously, we focused on gargle fluid as a noninvasive testing method and reported aberrant methylation in gargle fluid in patients with oral cancer. This study aimed to distinguish early-stage oral cancer from clinically diagnosed OPMDs using gargle fluid samples. We collected gargle fluid samples from 40 patients who were clinically diagnosed with OPMDs in the training set; among them, 9 patients were pathologically diagnosed with oral cancer. Methylation levels of 25 tumor suppressor genes were analyzed using the methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) method. We found that a combination of six genes (TP73, CASP8, RARB, KLLN, GSTP1, and CHFR) could distinguish oral cancer from clinically diagnosed OPMDs with high diagnostic performance (area under the curve [AUC], 0.885; sensitivity, 77.8%; and specificity, 87.1%). Additionally, the panel comprised of the six methylated genes was validated in the test set. Furthermore, when compared with cytology testing, the panel could accurately detect oral cancer. The present methylated gene panel may serve as a novel biomarker for oral cancer.\n\nKazuki Mori, Tomofumi Hamada, Mahiro Beppu, Hiroki Tsuchihashi, Yuichi Goto, Kenichi Kume, Hiroshi Hijioka, Keitaro Nishi, Yumiko Mishima and Tsuyoshi Sugiura\nDetecting Early-Stage Oral Cancer from Clinically Diagnosed Oral Potentially Malignant Disorders by DNA Methylation Profile\nCancers 2022, 14(11), 2646\nhttps://doi.org/10.3390/cancers14112646","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲総研第674号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"カゴシマ ダイガク","subitem_publisher_language":"ja-Kana"}]},"item_5_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_value":"【指導教員:杉浦剛】"}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2022-08-03"}]},"item_5_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"森, 和樹","creatorNameLang":"ja"},{"creatorName":"モリ, カズキ","creatorNameLang":"ja-Kana"},{"creatorName":"Mori, Kazuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-11-08"}],"displaytype":"detail","filename":"Diss_森_和樹_ISK674_2022.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"Diss_森_和樹_ISK674_2022","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/16255/files/Diss_森_和樹_ISK674_2022.pdf"},"version_id":"5566c567-65b2-44c7-bb19-11b9fc424756"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-10-27"}],"displaytype":"detail","filename":"Abstract_森_和樹_4518810389_2022.pdf","filesize":[{"value":"159.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Abstract_森_和樹_4518810389_2022","objectType":"abstract","url":"https://ir.kagoshima-u.ac.jp/record/16255/files/Abstract_森_和樹_4518810389_2022.pdf"},"version_id":"ad2be8fa-2e5a-42b5-9e21-6b1a09ed63c4"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-10-28"}],"displaytype":"detail","filename":"Result_MORI_Kazuki_isk_674.pdf","filesize":[{"value":"99.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Result_MORI_Kazuki_isk_674","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/16255/files/Result_MORI_Kazuki_isk_674.pdf"},"version_id":"7fcc342b-be19-473a-be89-2dc9885012ce"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-10-28"}],"displaytype":"detail","filename":"Comments_MORI_Kazuki_isk_674.pdf","filesize":[{"value":"56.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Comments_MORI_Kazuki_isk_674","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/16255/files/Comments_MORI_Kazuki_isk_674.pdf"},"version_id":"74c365cf-3c8c-4912-8d95-7314f1d046f6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"oral cancer","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"oral squamous carcinoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"oral potentially malignant disorders","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gargle fluid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA methylation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"biomarker","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Detecting Early-Stage Oral Cancer from Clinically Diagnosed Oral Potentially Malignant Disorders by DNA Methylation Profile","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Detecting Early-Stage Oral Cancer from Clinically Diagnosed Oral Potentially Malignant Disorders by DNA Methylation Profile","subitem_title_language":"en"},{"subitem_title":"臨床的に口腔潜在的悪性疾患と診断された病変から、早期癌を検出するDNAメチル化を指標とした検査法","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"18","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-10-27"},"publish_date":"2022-10-27","publish_status":"0","recid":"16255","relation_version_is_last":true,"title":["Detecting Early-Stage Oral Cancer from Clinically Diagnosed Oral Potentially Malignant Disorders by DNA Methylation Profile"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-09T02:08:08.414482+00:00"}