@phdthesis{oai:ir.kagoshima-u.ac.jp:00016267,
 author = {近藤, 清貴 and Kondo, Kiyotaka},
 month = {2022-12-16, 2022-11-22, 2022-12-16, 2022-12-16},
 note = {博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨, Asparagine-linked glycosylation (N-glycosylation) of proteins in the cancer secretome has been gaining increasing attention as a potential biomarker for cancer detection and diagnosis. Small extracellular vesicles (sEVs) constitute a large part of the cancer secretome, yet little is known about whether their N-glycosylation status reflects known cancer characteristics. Here, we investigated the N-glycosylation of sEVs released from small-cell lung carcinoma (SCLC) and non–small-cell lung carcinoma (NSCLC) cells. We found that the N-glycans of SCLC-sEVs were characterized by the presence of structural units also found in the brain N-glycome, while NSCLC-sEVs were dominated by typical lung-type N-glycans with NSCLC-associated core fucosylation. In addition, lectin-assisted N-glycoproteomics of SCLC-sEVs and NSCLC-sEVs revealed that integrin αV was commonly expressed in sEVs of both cancer cell types, while the epithelium-specific integrin α6β4 heterodimer was selectively expressed in NSCLC-sEVs. Importantly, N-glycomics of the immunopurified integrin α6 from NSCLC-sEVs identified NSCLC-type N-glycans on this integrin subunit. Thus, we conclude that protein N-glycosylation in lung cancer sEVs may potentially reflect the histology of lung cancers.

Kiyotaka Kondo, Yoichiro Harada, Miyako Nakano, Takehiro Suzuki, Tomoko Fukushige, Ken Hanzawa, Hirokazu Yagi, Koichi Takagi, Keiko Mizuno, Yasuhide Miyamoto, Naoyuki Taniguchi, Koichi Kato, Takuro Kanekura, Naoshi Dohmae, Kentaro Machida, Ikuro Maruyama, Hiromasa Inoue
Identification of distinct N-glycosylation patterns on extracellular vesicles from small-cell and non–small-cell lung cancer cells
Journal of Biological Chemistry (2022) 298(6) 101950
https://doi.org/10.1016/j.jbc.2022.101950},
 school = {鹿児島大学},
 title = {Identification of distinct N-glycosylation patterns on extracellular vesicles from small-cell and non-small-cell lung cancer cells},
 year = {},
 yomi = {コンドウ, キヨタカ}
}