{"created":"2023-07-25T08:15:22.219608+00:00","id":16675,"links":{},"metadata":{"_buckets":{"deposit":"dd98a754-aa27-4efa-8075-22b8607fc341"},"_deposit":{"created_by":18,"id":"16675","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"16675"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00016675","sets":["57:86"]},"author_link":["142906"],"item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2023-05-11","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2023-05-25"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Medical Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"We recently determined the RNA sequencing-based microRNA (miRNA) expression signature of colorectal cancer (CRC). Analysis of the signature showed that the expression of both strands of pre-miR-139 (miR-139-5p, the guide strand, and miR-139-3p, the passenger strand) was significantly reduced in CRC tissues. Transient transfection assays revealed that expression of miR-139-3p blocked cancer cell malignant transformation (e.g., cell proliferation, migration, and invasion). Notably, expression of miR-139-3p markedly blocked RAC-alpha serine/threonine-protein kinase (AKT) phosphorylation in CRC cells. A combination of in silico database and gene expression analyses of miR-139-3p-transfected cells revealed 29 putative targets regulated by miR-139-3p in CRC cells. RNA immunoprecipitation analysis using an Argonaute2 (AGO2) antibody revealed that KRT80 was efficiently incorporated into the RNA-induced silencing complex. Aberrant expression of Keratin 80 (KRT80) was detected in CRC clinical specimens by immunostaining. A knockdown assay using small interfering RNA (siRNA) targeting KRT80 showed that reducing KRT80 expression suppressed the malignant transformation (cancer cell migration and invasion) of CRC cells. Importantly, inhibiting KRT80 expression reduced AKT phosphorylation in CRC cells. Moreover, hexokinase-2 (HK2) expression was reduced in cells transfected with the KRT80 siRNAs or miR-139-3p. The involvement of miRNA passenger strands (e.g., miR-139-3p) in CRC cells is a new concept in miRNA studies. Our tumor-suppressive miRNA-based approach helps elucidate the molecular pathogenesis of CRC.\n\nRyutaro Yasudome, Naohiko Seki, Shunichi Asai, Yusuke Goto, Yoshiaki Kita, Yuto Hozaka, Masumi Wada, Kan Tanabe, Tetsuya Idichi, Shinichiro Mori and Takao Ohtsuka\nMolecular Pathogenesis of Colorectal Cancer: Impact of Oncogenic Targets Regulated by Tumor Suppressive miR-139-3p\nInternational Journal of Molecular Sciences 2022, 23, 11616\nhttps://doi.org/10.3390/ijms231911616","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲総研第703号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"カゴシマ ダイガク","subitem_publisher_language":"ja-Kana"}]},"item_5_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_value":"【指導教員:大塚隆生】"}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2023-01-04"}]},"item_5_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"安留, 龍太郎","creatorNameLang":"ja"},{"creatorName":"ヤスドメ, リュウタロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Yasudome, Ryutaro","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-06-16"}],"displaytype":"detail","filename":"Diss_安留_龍太郎_ISK703_2023.pdf","filesize":[{"value":"3.0 MB"}],"format":"application/pdf","licensefree":"Copyright © 2022 by the authors.\nLicensee MDPI, Basel, Switzerland.\nThis article is an open access article　distributed under the terms and　conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Diss_安留_龍太郎_ISK703_2023","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/16675/files/Diss_安留_龍太郎_ISK703_2023.pdf"},"version_id":"610880a2-4c83-4d8a-ab2c-023727bf2be5"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-06-16"}],"displaytype":"detail","filename":"Abstract_安留_龍太郎_4519810338_2023.pdf","filesize":[{"value":"38.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Abstract_安留_龍太郎_4519810338_2023","objectType":"abstract","url":"https://ir.kagoshima-u.ac.jp/record/16675/files/Abstract_安留_龍太郎_4519810338_2023.pdf"},"version_id":"4b0a6a02-5f48-4900-a421-93d3972d6e6b"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-06-05"}],"displaytype":"detail","filename":"Result_YASUDOME_Ryutaro_isk_703.pdf","filesize":[{"value":"130.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Result_YASUDOME_Ryutaro_isk_703","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/16675/files/Result_YASUDOME_Ryutaro_isk_703.pdf"},"version_id":"00037f09-2316-425e-997a-0916424e7a61"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-06-05"}],"displaytype":"detail","filename":"Comments_YASUDOME_Ryutaro_isk_703.pdf","filesize":[{"value":"62.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Comments_YASUDOME_Ryutaro_isk_703","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/16675/files/Comments_YASUDOME_Ryutaro_isk_703.pdf"},"version_id":"3ef85a38-ff74-4128-b331-89c215e4fce1"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"microRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"expression signature","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"miR-139-3p","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"tumor suppressor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"colorectal cancer","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"KRT80","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"HK2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"AKT","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Molecular Pathogenesis of Colorectal Cancer: Impact of Oncogenic Targets Regulated by Tumor Suppressive miR-139-3p","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Molecular Pathogenesis of Colorectal Cancer: Impact of Oncogenic Targets Regulated by Tumor Suppressive miR-139-3p","subitem_title_language":"en"},{"subitem_title":"大腸癌の分子病態 : 癌抑制型マイクロRNA（miR-139-3p）により制御される癌促進型遺伝子の影響","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"18","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-06-05"},"publish_date":"2023-06-05","publish_status":"0","recid":"16675","relation_version_is_last":true,"title":["Molecular Pathogenesis of Colorectal Cancer: Impact of Oncogenic Targets Regulated by Tumor Suppressive miR-139-3p"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-09T02:13:27.807350+00:00"}