@article{oai:ir.kagoshima-u.ac.jp:00001979,
 author = {Hasui, Kazuhisa and Sato, Eiichi and Tanaka, Yuetsu and Yashiki, Shinji and Izumo, Shuji},
 journal = {Dendritic cells},
 month = {Jan},
 note = {Lymph nodes of HTLV-I-associated non-neoplastic lymphadenopathy (HANNLA) and adult T-cell leukemia/lymphoma (ATLL) were examined by means of quantitative highly sensitive immunohistochemistry (the modified lmmunoMax) of HTLV-I p40tax and p27rex proteins in order to see the relation of HTLV-I infection and the pathogcnesis. Two cases of HANNLA showed follicular hyperplasia with follicle-lysis and paracortical hyperplasia. HTLV-I p40tax protein labeled by WATM-I and HTLV-I p27rex protein labeled by Rec-6 were seen dominantly in cytoplasm of paracortical lymphocytes in HANNLA and ATLL cells. In HANNLA cytoplasmic spotty stain of WATM-1 was seen in dendritic cells of the paracortex. Image analysis of the modified lmmunoMax indicated gradual increase of p40tax from HANNLA to ATLL and appeareance of the large amount of HTLV-I p27rex protein in the paracortical lymphocytes in HANNLA. Estimating dose of HTLV-I DNA forms by means of image analysis or polymerase chain reaction (PCR) for HTLV-I pX DNA and for human β-globin gene, increase of HTLV-I DNA forms in HANNLA suggested reproduction and reinfection of HTLV-I. Many LN-3 (Ia-like antigen)-positive dendritic cells in HANNLA suggested cxogenous antigen-presenting. These findings suggested a possibility of an explosive increase of HTLV-I-infected T-cells in the paracortex of HANNLA through the host immunity. Follicle-lysis in HANNLA might bc the expression of disordercd B-cell differentiation in germinal centers under the regulatory T-cells loss caused by HTLV-1-induced apoptosis.},
 pages = {19--27},
 title = {Quantitative Highly-Sensitive lmmunohistochemistry (Modified lmmunoMax) of HTLV-I p40tax and p27rex Proteins in HTLV-I-Associated Non-Neoplastic Lymphadenopathy (HANNLA) with Estimation of HTLV-I Dose by Polymerase Chain Reaction},
 volume = {7},
 year = {1997}
}